FastQCFastQC Report
Wed 25 May 2016
SRR1780116_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780116_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97552
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT4422645.33582089552239No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90839.310931605707726No Hit
ATTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT72047.384779399704773No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG69307.103903559127439No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC18771.9241020173855994No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC13671.4013039199606365No Hit
ATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTCTT11501.178858454977858TruSeq Adapter, Index 6 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC6380.6540101689355421No Hit
CTTATACACATCTCCGAGCCCACGAGACACAACAACATCTCGTATGCCGT5720.5863539445628998No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACACCATCTCGTATGCCGT4140.42438904379202885No Hit
CTTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG3270.33520583893718225No Hit
CTTTACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC3220.33008036739380026No Hit
ATCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC3130.3208545186157126No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGACGT2780.2849762178120387No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC2780.2849762178120387No Hit
TTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC2640.2706248974905691No Hit
TATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTCT2620.26857470887321633No Hit
CTTAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG2560.2624241430211579No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTAAGCCGT2510.25729867147777596No Hit
CCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG2270.2326964080695424No Hit
ATTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGACGT1820.18656716417910446No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGA1790.18349188125307528No Hit
CTAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT1700.1742660324749877No Hit
CTTATACACATCTCCGAGCCCACGAGACCCCACAACATCTCGTATGCCGT1550.15888961784484174No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGAATGCCGT1410.14453829752337216No Hit
CTTATACACATCTCCGAGCCCACGAGACAACACAACATCTCGTATGCCGT1320.13531244874528456No Hit
ATTATACACATCTCCGAGCCCACGAGACACAACAACATCTCGTATGCCGT1320.13531244874528456No Hit
ATTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTAAGCCGT1310.13428735443660816No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCACGTATGCCGT1260.1291618828932262No Hit
ATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTCAT1230.12608659996719698No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACAACTCGTATGCCGT1200.1230113170411678No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTAGTATGCCGT1190.12198622273249138No Hit
CTTATACACATCTCCGAGACCACGAGACACCACAACATCTCGTATGCCGT1160.1189109398064622No Hit
TCTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT1160.1189109398064622No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACCTCTCGTATGCCGT1140.11686075118910941No Hit
ATATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC1130.115835656880433No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCAGT1110.11378546826308021No Hit
GCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG980.10045924225028703No Hit
ATTATACACATCTCCGAGCCCACGAGACACCACACCATCTCGTATGCCGT980.10045924225028703No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACAT150.00221878970.000015
TAAACAC301.0144868E-770.000013
CTTTACA301.0144868E-770.000011
TATATAC150.00221878970.000013
AATCAGC150.00221878970.0000151
CAACTGC150.00221878970.0000151
ACGTATT150.00221878970.0000147
TCAACTG150.00221878970.0000150
CAACAGC150.00221878970.0000151
TTATATA150.00221878970.000012
GACATCT150.00221878970.0000149
GCAGTAA150.00221878970.0000146
CACCCCA150.00221878970.0000128
AAGACAC150.00221878970.0000124
GACGTAT150.00221878970.0000146
AGACGCC150.00221878970.0000125
AACTAGA150.00221878970.0000121
CTTATAT150.00221878970.000011
ATGCACA150.00221878970.000014
TAATAAA150.00221878970.000012
Produced by FastQC (version 0.11.5)