FastQCFastQC Report
Wed 25 May 2016
SRR1780115_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780115_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124672
Sequences flagged as poor quality0
Sequence length76
%GC92

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9679177.63651822381931No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG77366.205082135523614No Hit
TCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGT6630.531795431211499No Hit
ATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTGGTC4170.33447766940451745No Hit
CTCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGG3560.28554928131416835No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3280.26309034907597534No Hit
TCTTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGG2230.17886935318275154No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1920.1540041067761807No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1690.1355556981519507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCGC455.456968E-1270.011643
TTATGCA207.894449E-570.0116352
TAAACAT150.002218776170.0116355
AAACATC207.894449E-570.0116356
CAGTATC207.894449E-570.01163555
TATAAAC150.002218776170.0116353
TGGAGGG207.894449E-570.01163537
TTATAAA150.002218776170.0116352
GCAGCAC150.002218776170.01163527
GCAGTAT207.894449E-570.01163554
CGCAGTA150.002218776170.01163553
CTTATGC150.002218776170.0116351
CTTATAA150.002218776170.0116351
CGCAGCA150.002218776170.01163526
CTTGTGC301.0156327E-770.0116351
GCCGAAT150.002218776170.01163554
CGCCGAA150.002218776170.01163553
TGTCGGT207.894449E-570.01163543
GTTGTGC301.0156327E-770.0116351
CCGAATC150.002218776170.01163555
Produced by FastQC (version 0.11.5)