FastQCFastQC Report
Wed 25 May 2016
SRR1780115_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780115_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124672
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT6420151.49592530800822No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG116699.35976001026694No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG92187.393801334702259No Hit
ATTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT75596.063109599589323No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC28712.302842659137577No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC17961.4405800821355235No Hit
ATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTCTT17271.3852348562628336Illumina PCR Primer Index 10 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTC9750.7820521047227926No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG5550.44516812114989734No Hit
CTTTACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTC4910.3938334188911704No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC4000.3208418891170431No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACTAATCTCGTATGCCGT3870.3104145277207392No Hit
TTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTC3410.27351771047227924No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG3400.27271560574948667No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC3390.27191350102669404No Hit
TATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTCT3380.2711113963039014Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGACGT3330.2671008726899384No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG3070.24624614989733057No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCAGT2610.20934933264887065No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAAACTTATCTCGTATGCCGT2350.18849460985626282No Hit
CTAATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT2230.17886935318275154No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAACCTTATCTCGTATGCCGT2130.17084830595482545No Hit
CTTATACACATCTCCGAGACCACGAGACTTAAACTTATCTCGTATGCCGT2060.1652335728952772No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTATATCGTATGCCGT1860.14919147843942504No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAACTTATCTCGTATGCCGT1740.13956622176591377No Hit
TCTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT1640.13154517453798767No Hit
ATTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGACGT1590.12753465092402463No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACATATCTCGTATGCCGT1580.12673254620123203No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTAACTCGTATGCCGT1500.12031570841889118No Hit
CTTATACACATCTCCGAGCCAACGAGACTTAAACTTATCTCGTATGCCGT1450.11630518480492814No Hit
ATTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCAGT1400.11229466119096508No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGA1380.11069045174537986No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACC207.8827856E-570.0325228
AGCTCGT150.00221968470.0044237
AAATTAT353.6761776E-970.0044232
AAACACA150.00221968470.004424
CAGTATT150.00221968470.0044247
TATGCCT150.00221968470.0044243
TCGAATG500.070.0044240
CTCTCCG150.00221968470.0044210
ACACCTC150.00221968470.004426
AACCTTA252.8250852E-670.0044231
CTTAGCT150.00221968470.0044234
GAATGCC455.456968E-1270.0044242
CCGTATT252.8250852E-670.0044247
AACTTAG150.00221968470.0044232
CCTCTCC150.00221968470.004429
GCAGTAT150.00221968470.0044246
CACCTCT150.00221968470.004427
GCTCGTA150.00221968470.0044238
TACAAAT353.6761776E-970.004425
AGCGGGG150.00221968470.0044270
Produced by FastQC (version 0.11.5)