FastQCFastQC Report
Wed 25 May 2016
SRR1780114_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780114_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140291
Sequences flagged as poor quality0
Sequence length76
%GC80

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8331459.38656079149768No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG2845820.284979079199665No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT25191.795553528023893No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC16441.1718499404808576No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG15311.0913030771752998No Hit
TCTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG8340.594478619441019No Hit
CTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG3560.2537582596175093No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3270.233086940716083No Hit
CTTATACACATCTGACGCTGCCGACGAATGATGGAGTGTAGATCTCGGTG3130.22310768331539446No Hit
CTTTACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG2790.1988723439137222No Hit
CTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT2570.18319065371264015No Hit
TCTTAATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG1930.1375711913094924No Hit
CTTAATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT1800.12830473800885303No Hit
CTTATACACATCTGACGCTGCCGACGATTGAAGGAGTGTAGATCTCGGTG1740.12402791340855791Illumina Single End PCR Primer 1 (95% over 21bp)
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1640.1168998724080661No Hit
TATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGT1620.11547426420796772No Hit
CTTATACACATCTGACGCTGCCGACGATAGATGGAGTGTAGATCTCGGTG1580.11262304780777099No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCTC150.00222082270.0000125
GGGTCTG150.00222082270.000018
CGATATA150.00222082270.0000125
CCAACCC150.00222082270.0000135
CACACTC150.00222082270.0000114
AACAAAT150.00222082270.000017
GTGTCAT150.00222082270.0000157
CAGCATG150.00222082270.000014
GGCACCT150.00222082270.0000115
GTGCAGC150.00222082270.000011
GGGCACC150.00222082270.0000114
CACATAT252.8279992E-670.000017
CTTTACA301.01774276E-770.000011
TGCGCGT252.8279992E-670.000015
AAATCAC150.00222082270.0000110
AAACAAC150.00222082270.000016
TGCCGCT252.8279992E-670.0000113
CAACCCT150.00222082270.0000136
GGTCTGG150.00222082270.000019
CCGCTGC252.8279992E-670.0000115
Produced by FastQC (version 0.11.5)