##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780114_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 140291 Sequences flagged as poor quality 0 Sequence length 76 %GC 80 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.123942376916553 32.0 27.0 32.0 22.0 32.0 2 29.831464598584372 32.0 32.0 32.0 22.0 32.0 3 29.6608549372376 32.0 32.0 32.0 22.0 32.0 4 28.422364941443142 32.0 27.0 32.0 13.0 32.0 5 29.832712005759458 32.0 32.0 32.0 22.0 32.0 6 34.11128297609968 37.0 37.0 37.0 27.0 37.0 7 33.93002402149817 37.0 37.0 37.0 22.0 37.0 8 33.818156546036455 37.0 37.0 37.0 27.0 37.0 9 34.33277972214896 37.0 37.0 37.0 27.0 37.0 10-11 33.868640896422434 37.0 34.5 37.0 24.5 37.0 12-13 33.74220726917621 37.0 37.0 37.0 22.0 37.0 14-15 33.323042818142284 37.0 34.5 37.0 20.0 37.0 16-17 34.425394358868346 37.0 37.0 37.0 27.0 37.0 18-19 34.31634245960183 37.0 37.0 37.0 24.5 37.0 20-21 34.51725342324169 37.0 37.0 37.0 29.5 37.0 22-23 34.71943317817964 37.0 37.0 37.0 29.5 37.0 24-25 34.067324347249645 37.0 37.0 37.0 24.5 37.0 26-27 34.55180303797107 37.0 37.0 37.0 29.5 37.0 28-29 34.475041164436774 37.0 37.0 37.0 29.5 37.0 30-31 33.913558246787034 37.0 37.0 37.0 24.5 37.0 32-33 34.257122694969745 37.0 37.0 37.0 24.5 37.0 34-35 34.09352346194696 37.0 37.0 37.0 27.0 37.0 36-37 35.1927244085508 37.0 37.0 37.0 32.0 37.0 38-39 35.363961337505614 37.0 37.0 37.0 34.5 37.0 40-41 34.819072499305015 37.0 37.0 37.0 29.5 37.0 42-43 34.67460136430705 37.0 37.0 37.0 29.5 37.0 44-45 34.737901932411916 37.0 37.0 37.0 32.0 37.0 46-47 34.92199428331112 37.0 37.0 37.0 29.5 37.0 48-49 35.334796957752104 37.0 37.0 37.0 32.0 37.0 50-51 35.557313013664455 37.0 37.0 37.0 34.5 37.0 52-53 34.958030094589105 37.0 37.0 37.0 32.0 37.0 54-55 35.01100569530476 37.0 37.0 37.0 32.0 37.0 56-57 34.96919973483688 37.0 37.0 37.0 32.0 37.0 58-59 34.62522898831714 37.0 37.0 37.0 29.5 37.0 60-61 34.63180460614009 37.0 37.0 37.0 29.5 37.0 62-63 33.92684491521195 37.0 37.0 37.0 24.5 37.0 64-65 33.450128661140056 37.0 34.5 37.0 20.0 37.0 66-67 33.8130493046596 37.0 37.0 37.0 24.5 37.0 68-69 34.185186505192775 37.0 37.0 37.0 27.0 37.0 70-71 34.24872229865066 37.0 37.0 37.0 27.0 37.0 72-73 33.96965236544041 37.0 37.0 37.0 24.5 37.0 74-75 31.80647725085715 37.0 29.5 37.0 13.0 37.0 76 32.620324896108805 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.6052897729935793 11101 2 0.18861881921566592 11101 3 0.7799341507186028 11101 4 0.06366222967492874 11101 5 0.886253530519955 11101 6 -0.32243109246550006 11101 7 0.8885346235184173 11101 8 0.23822781336735233 11101 9 0.44909078861922325 11101 10-11 0.25443479369103983 11101 12-13 0.7054588856425212 11101 14-15 0.08062167862152592 11101 16-17 1.1373882657358365 11101 18-19 0.12826430925510124 11101 20-21 0.5956912588313301 11101 22-23 0.9641946479816781 11101 24-25 0.4858613465655637 11101 26-27 0.25962781116380285 11101 28-29 0.627930287775996 11101 30-31 0.2990508305950428 11101 32-33 0.7234341306556615 11101 34-35 -0.16304225504707404 11101 36-37 0.6565151304028163 11101 38-39 0.6181388219356592 11101 40-41 0.1821502201316889 11101 42-43 0.12344648559339788 11101 44-45 -0.17661409810659734 11101 46-47 0.6307190770865247 11101 48-49 0.12115604507381761 11101 50-51 0.5606623309305192 11101 52-53 0.5945524251734327 11101 54-55 0.0595878700460446 11101 56-57 0.1008150598158366 11101 58-59 0.11976414804883007 11101 60-61 0.44915319974372636 11101 62-63 0.2388799210394552 11101 64-65 -0.24806099640325385 11101 66-67 0.7417518989384391 11101 68-69 0.1362479302374595 11101 70-71 0.028767923323620437 11101 72-73 0.286419584783026 11101 74-75 0.9747358046703312 11101 76 1.1648735565825774 11102 1 -0.28703149796846716 11102 2 -0.6774202118719117 11102 3 -0.8190655550772199 11102 4 0.0056059386089302166 11102 5 -0.2678113905352575 11102 6 -1.2458399382044405 11102 7 -0.2523891775995537 11102 8 -0.3598892798953699 11102 9 -0.31358450803665505 11102 10-11 -0.32892305665711064 11102 12-13 -0.17627103492235108 11102 14-15 -1.357791580180077 11102 16-17 0.15366756356853273 11102 18-19 -0.686092854616625 11102 20-21 -0.30339670821781084 11102 22-23 -0.2030604270403913 11102 24-25 -0.08156044415719776 11102 26-27 -0.9191502608181494 11102 28-29 0.24904434092887584 11102 30-31 0.5428480258485493 11102 32-33 -0.08469571145476351 11102 34-35 0.1000743478753563 11102 36-37 0.23658770076664837 11102 38-39 -0.22671750835756654 11102 40-41 -0.5170750422003749 11102 42-43 0.048375386250455676 11102 44-45 -1.0919813629884274 11102 46-47 -1.0330055478031568 11102 48-49 -0.5083428271435011 11102 50-51 -0.3234702570855674 11102 52-53 -0.3355740826229834 11102 54-55 -0.8755891428008766 11102 56-57 -0.12969391341158598 11102 58-59 0.18723497280121393 11102 60-61 -0.09747816242161633 11102 62-63 0.21240154504651798 11102 64-65 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11105 4 -0.5291133522310894 11105 5 -0.18961383882567517 11105 6 -0.40455669633023206 11105 7 0.6727902229914093 11105 8 -0.6958960337999684 11105 9 1.005854073643384 11105 10-11 0.3199266689655147 11105 12-13 -0.3197387428159786 11105 14-15 -0.382432778998151 11105 16-17 -0.22059372811573041 11105 18-19 0.21291516564508584 11105 20-21 0.27569784644661155 11105 22-23 -0.4947974468799927 11105 24-25 -0.027423677589034412 11105 26-27 0.40065767501975813 11105 28-29 -0.17186110440670177 11105 30-31 -0.15686704380108552 11105 32-33 0.017351565877447683 11105 34-35 0.1937320093358892 11105 36-37 -0.09683357403284276 11105 38-39 -0.4303899439844088 11105 40-41 -0.05116115448403491 11105 42-43 -0.6194169311830677 11105 44-45 0.2472440485776346 11105 46-47 0.9800273774817754 11105 48-49 0.3191687811300312 11105 50-51 0.027943841690280635 11105 52-53 0.3231426755028082 11105 54-55 0.2287797892379615 11105 56-57 0.018469868774992904 11105 58-59 0.0473552767269112 11105 60-61 -0.3983435064486329 11105 62-63 0.4809747826995405 11105 64-65 -0.2043631483575794 11105 66-67 -0.7066451146759647 11105 68-69 -0.6007964263721135 11105 70-71 -0.7668513125130048 11105 72-73 -0.9579260454321741 11105 74-75 0.4931262306694606 11105 76 0.23481207217326983 11106 1 -0.8904040069107246 11106 2 0.6644295581842989 11106 3 0.5556917264761765 11106 4 -0.44181358106403934 11106 5 0.19013444492718534 11106 6 0.4471595508093529 11106 7 0.7886941131515925 11106 8 0.013028451325887147 11106 9 -0.5661138897433489 11106 10-11 -0.449924589615712 11106 12-13 -0.45434972679766616 11106 14-15 0.15069079079590608 11106 16-17 0.06710650071723023 11106 18-19 0.21119891850550232 11106 20-21 -0.04003441899961757 11106 22-23 -0.20709721804703207 11106 24-25 -0.2989339750718685 11106 26-27 -0.3149601739504959 11106 28-29 -0.2981196856424049 11106 30-31 0.4663022925833502 11106 32-33 -0.20564614579303253 11106 34-35 0.214841849152819 11106 36-37 -0.02503723536007385 11106 38-39 0.04466726425357592 11106 40-41 0.5244681361338195 11106 42-43 -0.04252905932951734 11106 44-45 0.1842577785547519 11106 46-47 -0.32861106645414395 11106 48-49 0.3036653486357537 11106 50-51 0.7508271368847943 11106 52-53 0.07668958625795597 11106 54-55 -0.16858863181467143 11106 56-57 0.07705110447293606 11106 58-59 -0.20075685141953414 11106 60-61 0.07339562398615129 11106 62-63 0.009693318168643827 11106 64-65 -0.7915485030486522 11106 66-67 -0.17781216273088774 11106 68-69 0.17048503815878746 11106 70-71 0.023823744695235405 11106 72-73 -0.2675942376518492 11106 74-75 -0.7541849821451905 11106 76 -0.23852888892513135 11107 1 0.6961988639026693 11107 2 0.7239723545691987 11107 3 0.5577119284963779 11107 4 0.1343693003819979 11107 5 0.11857676284318686 11107 6 -0.2113199813543929 11107 7 -0.2932835582997626 11107 8 0.1776217527612971 11107 9 -0.4397981002696625 11107 10-11 0.39079843005466586 11107 12-13 0.9680851482687842 11107 14-15 0.1715307695306194 11107 16-17 0.25860038694795406 11107 18-19 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11108 3 0.08973427966766678 11108 4 -0.13341492484903483 11108 5 -0.49049265473686887 11108 6 -0.5789322744985981 11108 7 -0.8200404893120137 11108 8 -0.4877554232541783 11108 9 0.7304146656877819 11108 10-11 -0.07288091660339546 11108 12-13 0.34133896301195676 11108 14-15 -0.1207537780730803 11108 16-17 0.4395589083320104 11108 18-19 0.08941840338791707 11108 20-21 0.41758036263532716 11108 22-23 0.46322752999328287 11108 24-25 0.4226225758799771 11108 26-27 0.6560924576284535 11108 28-29 0.5604469436969026 11108 30-31 0.1832115870962241 11108 32-33 0.49121835588669427 11108 34-35 -0.2548110063902911 11108 36-37 0.731681689611932 11108 38-39 0.19287555092770958 11108 40-41 0.5228443959322533 11108 42-43 0.038769932831741016 11108 44-45 -0.20476797732430896 11108 46-47 -0.1835942691417145 11108 48-49 0.20943242590338684 11108 50-51 0.5841418065376516 11108 52-53 -0.02907749100967294 11108 54-55 0.14899255519540588 11108 56-57 0.05616644601828824 11108 58-59 0.08054214074171995 11108 60-61 0.038289241007426256 11108 62-63 -0.20357655865106494 11108 64-65 0.8379587757867384 11108 66-67 0.7329941082657498 11108 68-69 0.4082230000848739 11108 70-71 0.10629854872658484 11108 72-73 0.3724393569730111 11108 74-75 1.3796895979219954 11108 76 1.5435539778161953 11109 1 -0.4856193179155106 11109 2 -0.21415895856211264 11109 3 -0.45112645534200624 11109 4 -0.6585599925472927 11109 5 0.4077181769846021 11109 6 -1.6683906884250987 11109 7 0.2483831083669088 11109 8 -0.2882873381477964 11109 9 -1.7352526457242092 11109 10-11 -0.5738480345917978 11109 12-13 -0.4024956598120255 11109 14-15 -0.8676106446107923 11109 16-17 0.2724892758368469 11109 18-19 -0.5800690240782274 11109 20-21 -1.2433238926838257 11109 22-23 -0.20625989747286866 11109 24-25 -0.0684315827273636 11109 26-27 -0.1791348150988199 11109 28-29 -0.2761101162644124 11109 30-31 -0.7470350279908189 11109 32-33 -0.46090930368777094 11109 34-35 -0.16619882070364156 11109 36-37 -0.25888891000122527 11109 38-39 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1.4767446315415 21611 28-29 1.0509649825498144 21611 30-31 1.2670459996771655 21611 32-33 1.2347428702252756 21611 34-35 1.378514618031538 21611 36-37 1.0499055564019386 21611 38-39 0.5315665777547167 21611 40-41 0.9379692802985744 21611 42-43 1.1197135035995416 21611 44-45 1.1168092659689393 21611 46-47 0.9202447687861266 21611 48-49 0.22134269417351504 21611 50-51 1.0225090590815924 21611 52-53 0.6981426755028082 21611 54-55 0.6798667457596963 21611 56-57 1.5076003035576022 21611 58-59 1.2593117984660438 21611 60-61 0.8407869283339764 21611 62-63 0.6711921740038846 21611 64-65 1.3988977212076392 21611 66-67 1.2063983635849027 21611 68-69 0.3992035736278936 21611 70-71 0.8418443396609092 21611 72-73 0.009465258915653862 21611 74-75 1.2757349263216327 21611 76 2.017420767825442 21612 1 -0.06451168991133116 21612 2 -0.5170586450824892 21612 3 -0.5983312620295713 21612 4 -1.3163447365389338 21612 5 -0.04930899124946109 21612 6 -0.582967490932937 21612 7 -1.082207278257961 21612 8 0.22464369633496517 21612 9 0.882040980190105 21612 10-11 -0.8345585884057911 21612 12-13 -0.9789716263741965 21612 14-15 -1.0921404251750602 21612 16-17 0.18301696653695387 21612 18-19 -0.7552257638901381 21612 20-21 -0.9638698696953227 21612 22-23 0.19042244317498813 21612 24-25 -0.6137555116719895 21612 26-27 -0.864022484900282 21612 28-29 -0.7160265216980548 21612 30-31 -1.40593001231683 21612 32-33 -0.4178058554119133 21612 34-35 -1.402157545886503 21612 36-37 -0.8922483666365437 21612 38-39 -0.21449789001140118 21612 40-41 -1.299911779171687 21612 42-43 -0.11566880524603107 21612 44-45 -1.1496200193883794 21612 46-47 -0.8624888643972781 21612 48-49 -0.17177324785546944 21612 50-51 -0.23017709783994889 21612 52-53 -0.5674120471358677 21612 54-55 -0.25110526823330304 21612 56-57 0.44250784978448365 21612 58-59 -0.49343682722111026 21612 60-61 -0.7702825369333866 21612 62-63 -0.8279957320430924 21612 64-65 -1.6491407595519831 21612 66-67 -0.8280843950357877 21612 68-69 -0.6481477507099385 21612 70-71 -0.3150147308038598 21612 72-73 -0.5801774197450129 21612 74-75 -0.5682805659322412 21612 76 -0.9956976729541687 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 3.0 11 12.0 12 54.0 13 94.0 14 185.0 15 295.0 16 498.0 17 836.0 18 969.0 19 1037.0 20 946.0 21 957.0 22 997.0 23 1133.0 24 1220.0 25 1440.0 26 1506.0 27 1654.0 28 1700.0 29 2145.0 30 2658.0 31 3453.0 32 4975.0 33 7904.0 34 14778.0 35 25082.0 36 63760.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 66.02704378755587 2.622406284080946 4.065121782580493 27.285428145782692 2 64.52944237335252 1.4099265098972849 29.718228539250557 4.342402577499626 3 64.32843161713866 2.871174914998111 30.40679729989807 2.393596167965158 4 65.5087856862815 24.994831949246176 6.8852692732651395 2.6111130912071854 5 65.01913879008632 6.193554825327356 28.022467585233553 0.7648387993527739 6 66.43048080692876 27.245250739565886 3.6204868660227394 2.7037815874826245 7 65.54019858722228 6.150073775224355 3.0935697942134563 25.216157843339914 8 66.36705134327933 27.244085507979843 2.2624402135561086 4.126422935184723 9 65.27147144150373 4.1898625000891005 0.9793928334675781 29.55927322493959 10-11 66.58124897534411 15.501707165819617 14.036181936118497 3.8808619227177794 12-13 65.86891439161737 3.4510656497255683 15.647587140922376 15.032432817734692 14-15 80.13878295827958 12.877875273538573 3.5722177473964836 3.4111240207853673 16-17 79.77268596234242 3.5009498148484752 1.6401680798058316 15.086196143003269 18-19 68.06914372271228 1.3622026196204224 13.256348569901096 17.312305087766195 20-21 79.89129466274615 1.7054263565891472 3.490332353203243 14.912946627461462 22-23 79.88245818498045 1.3603914734069662 0.8842366375485154 17.872913704064068 24-25 69.05626619430674 14.768138514561066 1.4651949375750686 14.710400353557135 26-27 80.1832658531022 14.200430542883211 2.055414575729927 3.5608890282846715 28-29 68.03574037971481 4.866722028377034 25.918190598726206 1.1793469931819558 30-31 80.53725959824929 13.253282579872547 5.085325691801035 1.124132130077128 32-33 80.34322373696872 4.555644658291009 13.946716564198521 1.1544150405417446 34-35 82.65502874433757 13.214197885072545 3.2148037793546873 0.9159695912352045 36-37 80.9396351090788 4.0855095036300195 14.150839163580764 0.8240162237104254 38-39 81.3333998631387 1.9092866104014599 15.844904767335766 0.9124087591240875 40-41 79.75208673400289 14.357656585240678 4.341689773399576 1.548566907356851 42-43 69.10024057738573 15.12786242537646 13.868306156999019 1.9035908402387953 44-45 67.88247285998389 2.309841686209379 15.6260914812782 14.18159397252853 46-47 79.43551836222628 1.0271726733576643 4.289390396897811 15.247918567518248 48-49 83.17847609068326 0.8368349959548222 13.86694038441662 2.1177485289452957 50-51 93.53130279205367 0.8200811171065856 3.9571319614230425 1.6914841294167124 52-53 70.41707357060882 1.1226984681403125 14.126754439114103 14.333473522136764 54-55 80.31114120749875 0.9818946468030508 2.813101432746454 15.893862712951742 56-57 79.67895188911581 1.728912506548911 2.037914185208549 16.55422141912674 58-59 69.0954711184452 13.77946160946906 14.337597165840393 2.7874701062453444 60-61 66.4670786733101 4.063754107592184 16.48596825125277 12.98319896784495 62-63 66.55879449281665 15.733180316417123 14.360661346715187 3.347363844051052 64-65 66.42062343624143 16.15046297946353 16.48549045884508 0.9434231254499704 66-67 66.43630557357417 30.61473978344394 2.0924960972862774 0.8564585456956098 68-69 66.49345280100364 31.841769490123955 0.8318542437397087 0.8329234651326903 70-71 66.70836630099295 31.62115347603875 0.8314978366087149 0.8389823863595862 72-73 68.14053946167883 30.19181968521898 0.8667883211678832 0.8008525319343065 74-75 77.54788534605048 20.731307428555134 0.9516477406385664 0.769159484755815 76 95.10934648722628 3.2390510948905114 0.9523266423357664 0.6992757755474452 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.5 14 1.5 15 4.0 16 6.0 17 4.0 18 8.5 19 9.5 20 15.5 21 25.0 22 21.0 23 24.0 24 29.0 25 27.0 26 38.0 27 60.0 28 80.0 29 89.0 30 104.5 31 123.5 32 137.0 33 147.0 34 165.5 35 192.0 36 238.0 37 276.0 38 280.0 39 374.5 40 556.0 41 1497.0 42 2347.0 43 7902.5 44 17905.0 45 12138.5 46 1925.0 47 1742.5 48 954.0 49 310.0 50 272.0 51 240.0 52 191.0 53 187.5 54 201.0 55 203.5 56 212.0 57 203.0 58 188.0 59 166.5 60 157.5 61 141.5 62 113.0 63 157.5 64 173.0 65 143.5 66 119.0 67 95.0 68 91.5 69 93.0 70 97.0 71 96.0 72 98.5 73 95.0 74 90.5 75 92.0 76 93.0 77 101.5 78 118.5 79 128.0 80 129.0 81 136.0 82 145.0 83 148.0 84 161.5 85 182.0 86 218.5 87 248.0 88 246.5 89 255.0 90 308.0 91 370.0 92 389.0 93 422.0 94 520.5 95 704.0 96 822.0 97 1034.0 98 1858.0 99 43371.5 100 84273.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.004276824600295101 5 0.0 6 0.004276824600295101 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.128041000491835E-4 14-15 0.0 16-17 3.5640205002459175E-4 18-19 0.0024948143501721423 20-21 0.0024948143501721423 22-23 3.5640205002459175E-4 24-25 0.0017820102501229587 26-27 0.0021384123001475506 28-29 0.0010692061500737753 30-31 0.0035640205002459174 32-33 0.0024948143501721423 34-35 0.0017820102501229587 36-37 0.0017820102501229587 38-39 0.0021384123001475506 40-41 0.0 42-43 0.0024948143501721423 44-45 0.0 46-47 0.0021384123001475506 48-49 3.5640205002459175E-4 50-51 0.0 52-53 0.002851216400196734 54-55 7.128041000491835E-4 56-57 3.5640205002459175E-4 58-59 0.0017820102501229587 60-61 0.001425608200098367 62-63 0.0010692061500737753 64-65 0.002851216400196734 66-67 0.002851216400196734 68-69 0.001425608200098367 70-71 0.001425608200098367 72-73 0.0021384123001475506 74-75 0.005702432800393468 76 0.0021384123001475506 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 140291.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 8.928584157216072 #Duplication Level Percentage of deduplicated Percentage of total 1 89.34216828995689 7.976990683650412 2 3.832029378891905 0.684291936047216 3 1.9319814785246687 0.5174957766357072 4 1.1655756027462878 0.41627759442872314 5 0.9500239501836181 0.4241184395292642 6 0.5668210122944276 0.3036545466209522 7 0.32731917611368355 0.20457477671411567 8 0.2235350471020278 0.1596681184110171 9 0.19160146894459523 0.15396568561062363 >10 1.1975091809037202 2.043609354841009 >50 0.11975091809037203 0.8033302207554297 >100 0.10378412901165576 1.9872978309371236 >500 0.007983394539358136 0.594478619441019 >1k 0.023950183618074404 4.05870654568005 >5k 0.0 0.0 >10k+ 0.01596678907871627 79.67153987069734 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 83314 59.38656079149768 No Hit CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG 28458 20.284979079199665 No Hit TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT 2519 1.795553528023893 No Hit ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC 1644 1.1718499404808576 No Hit CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG 1531 1.0913030771752998 No Hit TCTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG 834 0.594478619441019 No Hit CTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG 356 0.2537582596175093 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 327 0.233086940716083 No Hit CTTATACACATCTGACGCTGCCGACGAATGATGGAGTGTAGATCTCGGTG 313 0.22310768331539446 No Hit CTTTACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG 279 0.1988723439137222 No Hit CTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT 257 0.18319065371264015 No Hit TCTTAATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG 193 0.1375711913094924 No Hit CTTAATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT 180 0.12830473800885303 No Hit CTTATACACATCTGACGCTGCCGACGATTGAAGGAGTGTAGATCTCGGTG 174 0.12402791340855791 Illumina Single End PCR Primer 1 (95% over 21bp) GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 164 0.1168998724080661 No Hit TATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGT 162 0.11547426420796772 No Hit CTTATACACATCTGACGCTGCCGACGATAGATGGAGTGTAGATCTCGGTG 158 0.11262304780777099 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.002851216400196734 0.0 2 0.0 0.0 0.0 0.002851216400196734 0.0 3 0.0 0.0 0.0 0.002851216400196734 0.0 4 0.0 0.0 0.0 0.008553649200590202 0.0 5 0.0 0.0 0.0 0.014968886101032853 0.0 6 0.0 0.0 0.0 0.014968886101032853 0.0 7 0.0 0.0 0.0 0.014968886101032853 0.0 8 0.0 0.0 0.0 0.014968886101032853 0.0 9 0.0 0.0 0.0 0.014968886101032853 0.0 10 0.0 0.0 0.0 0.014968886101032853 0.0 11 0.0 0.0 0.0 0.014968886101032853 0.0 12 0.0 0.0 0.0 0.014968886101032853 0.0 13 0.0 0.0 0.0 0.01639449430113122 0.0 14 0.0 0.0 0.0 0.01639449430113122 0.0 15 0.0 0.0 0.0 0.01639449430113122 0.0 16 0.0 0.0 0.0 0.01639449430113122 0.0 17 0.0 0.0 0.0 0.01639449430113122 0.0 18 0.0 0.0 0.0 0.027799359901918157 0.0 19 0.0 0.0 0.0 0.038491421402655907 0.0 20 0.0 0.0 0.0 0.038491421402655907 0.0 21 0.0 0.0 0.0 0.038491421402655907 0.0 22 0.0 0.0 0.0 0.04847067880334448 0.0 23 0.0 0.0 0.0 0.04847067880334448 0.0 24 0.0 0.0 0.0 0.05916274030408223 0.0 25 0.0 0.0 0.0 0.06058834850418059 0.0 26 0.0 0.0 0.0 0.06130115260422978 0.0 27 0.0 0.0 0.0 0.06486517310447569 0.0 28 0.0 0.0 0.0 0.07056760590486917 0.0 29 0.0 0.0 0.0 0.07555723460521345 0.0 30 0.0 0.0 0.0 0.07912125510545936 0.0 31 0.0 0.0 0.0 0.08268527560570528 0.0 32 0.0 0.0 0.0 0.09337733710644304 0.0 33 0.0 0.0 0.0 0.10905902730752508 0.0 34 0.0 0.0 0.0 0.12117669700836119 0.0 35 0.0 0.0 0.0 0.1290175421089022 0.0 36 0.0 0.0 0.0 0.15040166511037772 0.0 37 0.0 0.0 0.0 0.17036017991175484 0.0 38 0.0 0.0 0.0 0.2238204874154436 0.0 39 0.0 0.0 0.0 0.24663021861701748 0.0 40 0.0 0.0 0.0 0.29581370152041114 0.0 41 0.0 0.0 0.0 0.3314539065228703 0.0 42 0.0 0.0 0.0 0.37778617302606726 0.0 43 0.0 0.0 0.0 0.439087325630297 0.0 44 0.0 0.0 0.0 0.4747275306327562 0.0 45 0.0 0.0 0.0 0.5060909110349203 0.0 46 0.0 0.0 0.0 0.5324646627367401 0.0 47 0.0 0.0 0.0 0.5730944964395435 0.0 48 0.0 0.0 0.0 0.6044578768417076 0.0 49 0.0 0.0 0.0 0.6415236900442651 0.0 50 0.0 0.0 0.0 0.660056596645544 0.0 51 0.0 0.0 0.0 0.7028248426484949 0.0 52 0.0 0.0 0.0 0.7391778517510033 0.0 53 0.0 0.0 0.0 0.7591363665523804 0.0 54 0.0 0.0 0.0 0.7726796444533149 0.0 55 0.0 0.0 0.0 0.7912125510545936 0.0 56 0.0 0.0 0.0 0.8083198494557741 0.0 57 0.0 0.0 0.0 0.8418216421580857 0.0 58 0.0 0.0 0.0 0.8845898881610367 0.0 59 0.0 0.0 0.0 0.9016971865622171 0.0 60 0.0 0.0 0.0 0.9230813095636926 0.0 61 0.0 0.0 0.0 0.9373373915646763 0.0 62 0.0 0.0 0.0 0.9551574940659059 0.0 63 0.0 0.0 0.0 0.9836696580678732 0.0 64 0.0 0.0 0.0 1.0079049974695455 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGCTC 15 0.002220822 70.00001 25 GGGTCTG 15 0.002220822 70.00001 8 CGATATA 15 0.002220822 70.00001 25 CCAACCC 15 0.002220822 70.00001 35 CACACTC 15 0.002220822 70.00001 14 AACAAAT 15 0.002220822 70.00001 7 GTGTCAT 15 0.002220822 70.00001 57 CAGCATG 15 0.002220822 70.00001 4 GGCACCT 15 0.002220822 70.00001 15 GTGCAGC 15 0.002220822 70.00001 1 GGGCACC 15 0.002220822 70.00001 14 CACATAT 25 2.8279992E-6 70.00001 7 CTTTACA 30 1.01774276E-7 70.00001 1 TGCGCGT 25 2.8279992E-6 70.00001 5 AAATCAC 15 0.002220822 70.00001 10 AAACAAC 15 0.002220822 70.00001 6 TGCCGCT 25 2.8279992E-6 70.00001 13 CAACCCT 15 0.002220822 70.00001 36 GGTCTGG 15 0.002220822 70.00001 9 CCGCTGC 25 2.8279992E-6 70.00001 15 >>END_MODULE