FastQCFastQC Report
Wed 25 May 2016
SRR1780114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780114_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140291
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4189929.865778987960738No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT3881027.66392712290881No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT80575.743062634096272No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG49743.545487593644639No Hit
CTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT47293.370850589132589No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC18281.3030058948899075No Hit
ATAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT17381.238853525885481No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC9150.6522157515450029No Hit
ATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCTT8410.5994682481413633RNA PCR Primer, Index 41 (95% over 22bp)
TCATATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG5540.3948934714272477No Hit
CTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC5070.36139167872493605No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT4360.310782587621444No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT4240.3022289384208538No Hit
CATATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT3640.25946069241790276No Hit
ATCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC3510.2501942391172634No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT3190.22738450791568954No Hit
ATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCAT3130.22310768331539446No Hit
CAAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT2930.20885160131441077No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACGT2860.20386197261406647No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCG2560.18247784961259098No Hit
CTAATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT2400.171072984011804No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACGT2370.1689345717116565No Hit
AATATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT2330.16608335531145976No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG2250.16038092251106628No Hit
TCTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG2240.1596681184110171No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT2160.15396568561062363No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTAAGCCGT2000.1425608200098367No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC1860.13258156260914813No Hit
AAAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT1820.12973034620895138No Hit
CTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACGT1780.12687912980875465No Hit
CTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTAAGCCGT1730.12331510930850874No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGCCATCTCGTATGCCGT1700.1211766970083612No Hit
CTTAAACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT1690.12046389290831201No Hit
TTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC1650.11761267650811527No Hit
CTTTACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC1620.11547426420796772No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAAAGACATCTCGTATGCCGT1580.11262304780777099No Hit
CCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG1580.11262304780777099No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG1540.10977183140757425No Hit
CTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT1450.10335659450713161No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCTT150.00222082270.0000153
CTCGAAA150.00222082270.0000139
TTATGAA150.00222082270.0000152
ACTAATA150.00222082270.000012
TCCTTAT252.8279992E-670.000011
AACCGAG150.00222082270.0000112
AATTCAG252.8279992E-670.0000150
TATGCTA150.00222082270.0000153
CAGTAGA150.00222082270.0000119
GCAGGGT150.00222082270.000013
TAACGAA150.00222082270.0000138
AGGGTTG150.00222082270.000015
AAGAGCC150.00222082270.0000114
TGCTGCA150.00222082270.0000139
CCATGCT150.00222082270.0000136
ATAACGA150.00222082270.0000137
AGAACAC150.00222082270.0000117
TGCCCTT150.00222082270.0000129
GGGAGCA150.00222082270.0000114
AGGTGCC150.00222082270.0000126
Produced by FastQC (version 0.11.5)