FastQCFastQC Report
Wed 25 May 2016
SRR1780113_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780113_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281024
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6449322.949285470280117No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG207267.3751708039171024No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT18520.6590184468230471No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC13520.481097699840583No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG13320.4739808699612844No Hit
TCTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG7210.2565617171487133No Hit
CTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG2980.10604076520154863No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGGT150.002222321270.00658457
GCGTGTC150.002222321270.00658457
ATCCTTG207.9215024E-569.9941345
AAAAAGG20900.055.9382870
GCCGTAT30050.053.4564154
TCGCCGT30150.053.0469652
CGCCGTA30400.052.9560953
GTCGCCG30300.052.78434851
GATCTCG30250.052.75590541
CGTATCA30500.052.6770956
CTCGGTG30400.052.6107244
ATCTCGG30350.052.58207742
TCGGTGG30550.052.58151645
TGGTCGC30500.052.5529749
TGCGGGC200.006944014752.5049419
CCCCGTA200.006944014752.5049437
CCCGTAA200.006944014752.5049438
CCCCCGT200.006944014752.5049436
CTATGTT200.00694890452.49559852
ACGCCGT200.00694890452.49559852