FastQCFastQC Report
Wed 25 May 2016
SRR1780113_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780113_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281024
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3075310.94319346390344No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT2970010.56849237075837No Hit
ATTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT76332.7161381234342974No Hit
CTAATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT41321.4703370530630837No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG40381.4368879526303804No Hit
ATAATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT18860.6711170576178548No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC17720.6305511273058528No Hit
ATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTCTT10470.3725660441812799Illumina PCR Primer Index 5 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC8090.2878757686176269No Hit
TCATATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG5420.1928660897289911No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC5280.18788430881348214No Hit
CTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTC3850.1369989751764974No Hit
CATATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT3180.11315759508084718No Hit
CAAATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT2850.10141482578000455No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGC150.002223890569.99412541
AGGGGGG54300.064.4513170
ATTATAC9750.059.236341
CCAGGAC308.367855E-658.3284383
TGCCGAC553.6379788E-1157.26792545
CGCAACC252.386809E-456.0052718
TGACGAC252.3889092E-455.995345
AAGGGGG62550.055.55888769
ATCTCGT63750.055.4463337
CGTATGA1200.055.41201841
GATCTCG64250.055.39613336
CGTCTTC64300.055.18977448
TGATCTC64000.055.17505635
TAATACA11800.055.1746832
GCCGTCT64150.055.100646
CCGTCTT63600.055.0818647
TATGCCG64300.055.08091743
TCTCGTA64600.055.04182438
TTGATCT63550.054.96976534
TGCCGTC64400.054.94104845
Produced by FastQC (version 0.11.5)