FastQCFastQC Report
Wed 25 May 2016
SRR1780112_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780112_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107542
Sequences flagged as poor quality0
Sequence length76
%GC80

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6393059.446541816220645No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG2305921.441855275148313No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT22582.099644789942534No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG13381.2441650703911031No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC7520.6992616838072567No Hit
TCTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG6930.6443993974447193No Hit
CTTATACACATCTGACGCTGCCGACGAATGATGGAGTGTAGATCTCGGTG2540.23618679213702554No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2230.20736084506518385No Hit
CTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT1820.16923620538952222No Hit
TCTTAATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG1720.1599375127857023No Hit
CTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG1670.15528816648379237No Hit
CTTTACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG1550.14412973535920848No Hit
CTTATACACATCTGACGCTGCCGACGATAGATGGAGTGTAGATCTCGGTG1210.11251418050622082No Hit
CTTAATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT1190.11065444198545685No Hit
CTTATACACATCAGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG1080.10042588012125495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGTA252.8235008E-670.0000134
TCTAGGT252.8235008E-670.0000143
CTAGGTG252.8235008E-670.0000144
CTTTTAT150.002219407670.01
CGCTGCG150.002219407670.018
CTATACA301.015469E-770.01
CACATAA207.8960205E-570.07
CATCTGG150.002219407670.09
TAGCCGT207.8960205E-570.052
TTGAGTG207.8960205E-570.032
TTTTATA150.002219407670.02
AGGTGGT353.6707206E-970.046
ATTGAGT207.8960205E-570.031
GGTAGCC207.8960205E-570.050
GGTGGTA207.8960205E-570.047
GTGGTAG207.8960205E-570.048
AGCCGTA207.8960205E-570.053
ATCTGGC207.8960205E-570.010
TGAGTGT207.8960205E-570.033
GTAGTGT207.8960205E-570.033
Produced by FastQC (version 0.11.5)