FastQCFastQC Report
Wed 25 May 2016
SRR1780112_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780112_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107542
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3284230.538766249465326No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT3011928.006732253445165No Hit
ATTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT78397.289245132134421No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG44294.118390954231835No Hit
CTAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT43284.024474158933254No Hit
ATAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT19731.8346320507336669No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC16501.534284279630284No Hit
ATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTCTT10570.9828718082237637TruSeq Adapter, Index 8 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC7820.7271577616187164No Hit
TCATATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG6100.567220248833014No Hit
CTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTC4740.44075802942106335No Hit
ATCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC3960.3682282271112682No Hit
CAAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT3830.3561399267263023No Hit
CATATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT3730.34684123412248236No Hit
AATATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT3350.3115062022279667No Hit
AAAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT2630.24455561548046345No Hit
TCTAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG2270.2110803221067118No Hit
CTTAAACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT2150.19992189098212793No Hit
TTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTC1670.15528816648379237No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT1660.15435829722341038No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC1660.15435829722341038No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATTTAATCTCGTATGCCGTC1650.15342842796302839No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG1640.15249855870264642No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG1610.14970895092150044No Hit
TCAAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG1570.14598947387997246No Hit
CTAAAACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT1500.13948038905729854No Hit
CTTTACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTC1420.13204143497424262No Hit
TATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTCT1260.11716352680813076No Hit
ATATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTC1190.11065444198545685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGAAA252.8235008E-670.0000127
ATGACGA252.8235008E-670.0000144
CACAACA252.8235008E-670.000017
TTACATC252.8235008E-670.0000133
CAACACC252.8235008E-670.000019
TACACAA1000.070.000015
GTAAGCA252.8235008E-670.0000142
GACGACT252.8235008E-670.0000146
CTTAAAC455.456968E-1270.000011
ACGACTT252.8235008E-670.0000147
AATATAC1000.070.000011
CAGAAAT252.8235008E-670.0000128
TCTTTAT900.070.000011
TTTACAC252.8235008E-670.000012
TGACGAC252.8235008E-670.0000145
ACAACAC252.8235008E-670.000018
AACACCG252.8235008E-670.0000110
AGGGGTG150.002219407670.070
GAGAACA150.002219407670.016
CACATAA150.002219407670.07
Produced by FastQC (version 0.11.5)