FastQCFastQC Report
Wed 25 May 2016
SRR1780111_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780111_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188131
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6182632.86327080598094No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG154728.224056641382866No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT13470.7159904534606205No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC10620.564500268429977No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG9760.5187874406663442No Hit
TCTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG4880.2593937203331721No Hit
CTTATACACATCTGACGCTGCCGACGAATGATGGAGTGTAGATCTCGGTG2530.13448076074650112No Hit
CTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG2520.13394921623762165No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTC207.91246E-569.9981424
TGCGCAC150.002222239369.9981419
ATCCCCC150.002222239369.998146
AATTACG150.002222239369.998147
GTATACG150.002222239369.9981468
TATACGG150.002222239369.9981469
GCATCCC150.002222239369.9981450
TACGTAA150.002222239369.9981410
GCGCGTC308.3543055E-658.331786
GGTCACC252.3830617E-456.013431
GGTCGCA252.3861954E-455.9985152
TGGCGGC252.3861954E-455.9985119
AAAAAGG17950.055.56955370
CCGTGGT200.006936478452.5125630
CTAGATT200.00694377752.49860457
CGAGTTC200.00694377752.49860460
ATTACGT200.00694377752.4986048
TGGCGAC200.00694377752.49860419
CTGCGCA200.00694377752.49860418
AATGCAC200.00694377752.49860441
Produced by FastQC (version 0.11.5)