FastQCFastQC Report
Wed 25 May 2016
SRR1780111_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780111_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188131
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2437212.954802770409982No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT2350012.491295958667099No Hit
ATTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT93674.9789774146738175No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG44622.3717515986201105No Hit
CTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT29101.5467945208392024No Hit
ATAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT21421.1385683380197842No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC11240.5974560279805029No Hit
ATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCTT9990.5310129643705715TruSeq Adapter, Index 18 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC8700.4624437227251224No Hit
CTTATACACATCTCCGAGCCCACGAGACACGGGCGCATCTCGTATGCCGT5830.30989044867671994No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC5670.30138573653464873No Hit
TCATATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG5660.3008541920257693No Hit
AATATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT3570.189761389669964No Hit
CTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC3130.16637343127926818No Hit
CATATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT2530.13448076074650112No Hit
TCTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG2360.12544450409555044No Hit
AAAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT2290.1217236925333943No Hit
CAAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT2270.12066060351563537No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGAGCATCTCGTATGCCGT2200.11693979195347923No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT2010.10684044628476966No Hit
ATTATACACATCTCCGAGCCCACGAGACACGGGCGCATCTCGTATGCCGT1990.10577735726701075No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACGT150.00222344469.9885739
ACTCCGA600.069.9885711
TCGAAAG353.6961865E-969.9885740
GGAGCAT850.065.9066332
GAATCTC850.065.871635
AATCTCG800.065.6142836
AACTCCG750.065.3226710
GCGAATC650.064.6391933
ATTATAC12200.064.538641
CGAATCT600.064.19031534
AGGGGGG53000.063.451970
ATAATAC3900.062.8102531
GGGAGCA850.061.78746831
CGTCTAC750.060.6567648
CGAATGC650.059.22109241
TCTCGAA950.058.93774438
AACTCGT900.058.32380737
CAGCATG553.6379788E-1157.26337454
AATACAC11650.056.7718433
AGAAACG252.3784097E-456.0355425
Produced by FastQC (version 0.11.5)