FastQCFastQC Report
Wed 25 May 2016
SRR1780110_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780110_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292924
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5775719.717401100626784No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG215627.360953694473651No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT18860.6438530130682362No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG13460.45950485450150896No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC13050.44550804986959075No Hit
TCTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG6460.2205350193224181No Hit
CTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG3200.10924335322472724No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGCG150.002223094270.00122
CGACCTA150.002224600269.9892414
ATCGAGC150.002224600269.9892435
AAAAAGG22200.055.3387870
AACGTTT200.006941731652.5098657
ACGTTTG200.006941731652.5098658
AAACGTT200.006941731652.5098656
CAACGAA200.00694642152.50089620
TGCGGGC200.00694642152.50089619
CGAGCCT200.006951113752.4919368
TCTCGGT33400.052.39610743
CTCGGTG33500.052.3352444
TCGCCGT32900.052.22601752
CGGTGGT33600.052.17948546
TGGTCGC33350.052.1509149
GCCGTAT33050.052.10376754
AGATCTC33800.052.0866940
TCGGTGG33800.052.07779745
GTCGCCG33200.052.07031251
ATCTCGG33750.052.06014642