FastQCFastQC Report
Wed 25 May 2016
SRR1780110_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780110_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292924
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3194410.905217735658395No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT253628.658218514017287No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT64992.2186642268984444No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCG32031.0934576886837541No Hit
CTAATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT29080.9927489724297087No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC17200.5871830235829089No Hit
ATAATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT14090.4810121396676271No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC6020.2055140582540181No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCGT5420.18503092952438174No Hit
ATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTCTT5340.18229984569376356RNA PCR Primer, Index 26 (95% over 21bp)
ATCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC3960.13518864961559995No Hit
TCATATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCG3470.11846076115306359No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCGT3470.11846076115306359No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGACGT3060.10446395652114543No Hit
CTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTC3040.10378118556349086No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCG150.002220234870.0239129
TTCGATC150.002220234870.0239128
GGTCCGT150.002223244970.01
TTCCGTA150.002223244970.045
CACAACG150.002223244970.010
TAAGACG150.002223244970.043
GATCGAT150.00222475169.9880531
GTCTAGC150.00222475169.988052
TCGATTT150.00222475169.9880533
AGCCGTC600.064.16666445
AGGGGGG45400.063.83260370
CCGACTT550.063.6363647
GACGTAC501.4551915E-1163.0215226
CGACTTC501.4551915E-1162.98924648
CGTACGA453.8198777E-1062.24347728
ACGTACG453.8198777E-1062.24347727
TCTTAAG1700.061.7541650
GCCGAAA401.0635631E-861.25000446
AAGCCGT1050.059.99999644
ATTATAC10150.059.9999961
Produced by FastQC (version 0.11.5)