FastQCFastQC Report
Wed 25 May 2016
SRR1780109_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780109_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137733
Sequences flagged as poor quality0
Sequence length76
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6174244.827310811497604No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG2037714.794566298563161No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT18381.334465959501354No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC13300.965636412479217No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG12720.9235259523861384No Hit
TCTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG5660.4109400071152157No Hit
CTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG2700.19603145215743503No Hit
CTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT2230.16190745863373338No Hit
CTTTACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG2110.1531949496489585No Hit
CTTATACACATCTGACGCTGCCGACGAATGATGGAGTGTAGATCTCGGTG2100.1524689072335606No Hit
TCTTAATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG1670.12124908337145054No Hit
CTTAATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT1550.11253657438667568No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAAAC150.002220736470.0000151
CGCTCTT150.002220736470.0000117
ACCTACC150.002220736470.0000168
TAGGAGC150.002220736470.000014
AAGGGGG1450.062.7586270
TCTTTAT900.062.2222181
AAAAAGG18950.057.07123670
TGTGGCG252.383628E-455.99999611
TAACCTA252.383628E-455.9999966
CTATACA452.6802809E-854.4444431
AGATCTC29850.052.88107340
GATCTCG29900.052.7926441
TCTCGGT30000.052.73333443
ATCTCGG29950.052.7045142
GTATCAT29950.052.5876557
TAGATCT29950.052.5876539
GTCGCCG30100.052.55813651
CGTATCA30050.052.52911856
GCGAAGT200.006939115552.538
ACCGCAA200.006939115552.58