FastQCFastQC Report
Wed 25 May 2016
SRR1780109_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780109_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137733
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3031322.00852373795677No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT2852020.70672968714832No Hit
ATTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT81035.883121691969245No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG40882.968061394146646No Hit
CTAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT36702.6645756645103207No Hit
ATAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT18561.3475347229785164No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC17391.2625877603769613No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC8780.6374652407193628No Hit
ATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTCTT8760.636013155888567RNA PCR Primer, Index 39 (95% over 22bp)
ATCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC5070.36810350460673913No Hit
TCATATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG4900.3557607835449747No Hit
CTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTC4390.3187326203596814No Hit
CATATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT3570.259197142297053No Hit
AATATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT2960.21490855495778063No Hit
CAAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT2850.20692208838840365No Hit
AAAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT2130.1546470344797543No Hit
TCTAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG2050.14883869515657105No Hit
CTTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG1970.14303035583338777No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC1740.1263313802792359No Hit
TATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTCT1720.12487929544844009RNA PCR Primer, Index 39 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTC1660.12052304095605267No Hit
CTTAAACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT1510.10963240472508405No Hit
CTTAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG1500.10890636230968613No Hit
TTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTC1430.10382406540190077No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCA150.002215756870.03960420
CTATCGC150.002215756870.03960423
CGTTCAT150.002215756870.03960421
CATTTTG150.002215756870.03960425
GCGAACA150.002222153169.988758
TTTCGTT150.002222153169.9887518
GACGTAG150.002222153169.988756
TTCCGTC150.002222153169.9887545
CGTCTAA401.3460522E-1069.9887548
GTCTAAA207.9101956E-569.9887549
CCGTCGT150.002222153169.9887547
GACTTAT207.9101956E-569.9887549
ATTCCGT150.002222153169.9887544
CCGTAAT207.9101956E-569.9887547
ATTTTCG150.002222153169.9887516
CAAGGGG150.002222153169.9887568
TGTTATA150.002222153169.9887544
GTCGTCT150.002222153169.9887549
CGAATGC500.069.9887541
ATGACGA252.8304275E-669.9887544
Produced by FastQC (version 0.11.5)