FastQCFastQC Report
Wed 25 May 2016
SRR1780108_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780108_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123103
Sequences flagged as poor quality0
Sequence length76
%GC81

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7609961.817339951097864No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG2474020.09699194983063No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT22331.8139281739681405No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC13941.132385075912041No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG12140.9861660560668709No Hit
TCTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG7920.6433636873187493No Hit
CTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG3000.24369836640861717No Hit
CTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT2560.20795593933535333No Hit
CTTATACACATCTGACGCTGCCGACGAATGATGGAGTGTAGATCTCGGTG2540.2063312835592959No Hit
CTTTACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG2400.19495869312689373No Hit
TCTTAATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG1760.14296970829305541No Hit
CTTAATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT1720.1397203967409405No Hit
CTTATACACATCTGACGCTGCCGACGATAGATGGAGTGTAGATCTCGGTG1650.13403410152473944No Hit
TATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGT1510.1226615110923373No Hit
CTTATACACATCTGACGCTGCCGACGATTGAAGGAGTGTAGATCTCGGTG1460.1185998716521937Illumina Single End PCR Primer 1 (95% over 21bp)
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1370.11128892065993518No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGTA207.9005724E-570.034
ATAAGAC150.002220175270.010
ATGACGC207.9005724E-570.012
TATCGGT207.9005724E-570.043
CGCTCAC150.002220175270.016
TATGACG150.002220175270.011
CCCATCC150.002220175270.016
CGGGCTA150.002220175270.025
ACATATG207.9005724E-570.08
ATCGGTG207.9005724E-570.044
AGAGTAG150.002220175270.035
GACGCCG150.002220175270.051
ATAGATC150.002220175270.027
AAGGTGG150.002220175270.045
TGGACGC150.002220175270.049
ATATGAC150.002220175270.010
ACCCATC150.002220175270.015
CTTGTGC353.6761776E-970.01
CTTAAAC150.002220175270.01
AAGGAGT150.002220175270.031
Produced by FastQC (version 0.11.5)