FastQCFastQC Report
Wed 25 May 2016
SRR1780108_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780108_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123103
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT3779830.704369511709707No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3607029.300666921196072No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT82206.67733523959611No Hit
CTAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT49884.051891505487275No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG49314.005588815869637No Hit
ATAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT20851.6937036465398891No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC16571.3460273104635954No Hit
ATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTCTT11550.9382387106731761Illumina PCR Primer Index 3 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC10710.8700031680787633No Hit
TCATATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG6300.5117665694580961No Hit
CTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTC5270.4280967969911375No Hit
CATATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT4640.37692014004532787No Hit
CAAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT4510.3663598775009545No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC4030.3273681388755757No Hit
AATATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT3130.2542586289529906No Hit
TCTAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG2770.22501482498395653No Hit
AAAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT2680.21770387399169802No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG2290.18602308635857778No Hit
CTTAAACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT2230.18114911903040543No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC1920.155966954501515No Hit
TTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTC1790.14540669195714157No Hit
TATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTCT1770.14378203618108412No Hit
CTTTACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTC1690.13728341307685435No Hit
CTAAAACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT1680.1364710851888256No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG1570.12753547842050966No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCAGCCATCTCGTATGCCGT1460.1185998716521937No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG1450.11778754376416496No Hit
TCTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT1430.11616288798810752No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGACGT1430.11616288798810752No Hit
TCAAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG1400.11372590432402135No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGAAGACATCTCGTATGCCGT1380.1121012485479639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGA252.820485E-670.0227626
AATCGAA252.820485E-670.0227627
ATCGAAG252.820485E-670.0227628
GAGAATC207.887853E-570.0227524
CTATAAG150.002217313770.0227528
ATAAGAC150.002217313770.0227530
AGAATCG207.887853E-570.0227525
CGAGAAT207.887853E-570.0227523
AGACTAT150.002217313770.0227525
ACAACGA150.002217313770.0227519
GACTATA150.002217313770.0227526
ACTATAA150.002217313770.0227527
TTATGAT207.903752E-569.99431652
CTATATA207.903752E-569.9943162
TAAACAA207.903752E-569.9943165
ACTTGAA207.903752E-569.99431656
TATGATT207.903752E-569.99431653
TCTATAT207.903752E-569.9943161
TCAGCTA207.903752E-569.99431653
CATTATA207.903752E-569.9943162
Produced by FastQC (version 0.11.5)