FastQCFastQC Report
Wed 25 May 2016
SRR1780107_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780107_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161003
Sequences flagged as poor quality0
Sequence length76
%GC75

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8313051.63257827493898No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG2269314.094768420464215No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT22131.374508549530133No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC13410.8329037347130178No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG12330.7658242392998889No Hit
TCTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG6570.40806693042986775No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATC4600.2857089619448085No Hit
CTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG2830.17577312223995828No Hit
CTTATACACATCTGACGCTGCCGACGAATGATGGAGTGTAGATCTCGGTG2680.16645652565480146No Hit
CAGCTGTCTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAG2590.16086656770370739No Hit
CTGCTGTCTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAG2270.1409911616553729No Hit
CTTTACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG2090.12981124575318473No Hit
CTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT2050.1273268199971429No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGCT150.002218958770.0195732
ATTCCGC150.002218958770.0195731
GATTCCG150.002218958770.0195730
ATTGAAC207.897013E-570.0195727
CTAGGTC207.897013E-570.0195733
GTGCCCT150.002218958770.0195732
ATGGGAT150.00222169569.99782651
GTTCTTA150.00222169569.9978261
CGAACAG150.00222169569.99782614
AGTTTGT150.00222169569.99782669
ACTGAGT207.909178E-569.9978265
CTCCCCC150.00222169569.9978269
GTCTACT207.909178E-569.9978261
TTTCCGA150.00222169569.99782665
TAGCCTC207.909178E-569.99782611
CCCAATG150.00222169569.99782643
AAGTTTG150.00222169569.99782668
TTCCAAC150.00222169569.99782670
CGCTACG150.00222169569.99782635
ACAGCAT150.00222169569.99782617
Produced by FastQC (version 0.11.5)