FastQCFastQC Report
Wed 25 May 2016
SRR1780107_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780107_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161003
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT3777123.459811307863827No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3367420.915138227238No Hit
ATTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT108106.714160605702999No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG55543.449625162264057No Hit
CTAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT50023.106774407930287No Hit
ATAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT24871.544691713818997No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC17791.1049483549995962No Hit
ATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTCTT11530.7161357241790525TruSeq Adapter, Index 3 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC9890.6142742681813382No Hit
TCATATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG6720.41738352701502457No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC6460.4012347596007527No Hit
CTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC4980.3093110066272057TruSeq Adapter, Index 3 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT4320.2683179816525158No Hit
CAAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT3940.2447159369701186No Hit
AATATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT3680.2285671695558468No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTA3280.20372291199542866No Hit
AAAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT3200.198754060483345No Hit
CTTATACACATCTCCGAGCCCACGAGACAACTAGGCATCTCGTATGCCGT3160.19626963472730322No Hit
TCTAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG2950.1832263995080837No Hit
TTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC2300.14285448097240425TruSeq Adapter, Index 3 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTCT2120.13167456507021608RNA PCR Primer, Index 3 (95% over 22bp)
CTTAAACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT1940.12049464916802793No Hit
CTTAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG1770.10993583970485023No Hit
CTTTACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC1720.10683030750979795RNA PCR Primer, Index 3 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC1720.10683030750979795No Hit
CTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTC1710.1062092010707875No Hit
CTTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG1680.10434588175375614No Hit
ATATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC1640.10186145599771433TruSeq Adapter, Index 3 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACAC455.456968E-1270.017712
CACTAGT150.002219193170.0177129
AGGCTAA150.002219193170.017716
TCTTTAT1150.070.01771
TTGATGC150.002221929869.99596439
GTATTCT455.456968E-1269.99596449
CGTGCTT150.002221929869.99596434
CAGTTGG150.002221929869.99596432
TCTTAAG150.002221929869.99596450
TAACGTA207.9102174E-569.99596438
GCGTTTT150.002221929869.99596413
TGTGAAG150.002221929869.99596447
TAACCGA150.002221929869.99596411
TCTAATG207.9102174E-569.99596450
CATAACG207.9102174E-569.99596436
ACGTATG750.069.99596440
ATAACCG150.002221929869.99596410
CACGTAT550.069.99596439
AATGTCT150.002221929869.99596415
ACGGTCT150.002221929869.99596445
Produced by FastQC (version 0.11.5)