FastQCFastQC Report
Wed 25 May 2016
SRR1780106_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780106_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196752
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5591928.421057981621534No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG168308.55391558916809No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT14910.7578067821419858No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG11310.5748353256891925No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC10960.5570464340896153No Hit
TCTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG5740.29173782223306494No Hit
CTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG2580.13112954379116856No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGAGG150.002222604869.9964453
CATCGCC150.002222604869.99644537
AAAAAGG18500.055.80797270
CCGATGT200.006937936452.51067415
TCGATTG200.006944915752.4973369
GCACAGT200.006944915752.4973332
ACTCGAT200.006944915752.4973367
CATGTAG200.006944915752.497332
CACGTCT200.006944915752.497337
ACAAGTT200.006944915752.4973339
TCGCCGT25600.052.10043352
GTCGCCG25700.052.03392451
AGATCTC25500.051.87971540
TGGTCGC25800.051.83224549
GCCGTAT25600.051.8137754
TCTCGGT25550.051.7781943
TAGATCT25700.051.74834439
CTCGGTG25600.051.6770644
CGCCGTA25700.051.62528253
TCGGTGG25700.051.61216445