FastQCFastQC Report
Wed 25 May 2016
SRR1780106_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780106_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196752
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2523212.824266081158006No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT2425512.327701878506952No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT80144.073147922257461No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG36681.8642758396356836No Hit
CTAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT31371.5943929413678133No Hit
ATAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT17410.8848702935675369No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC14350.7293445555826624No Hit
ATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTCTT8550.43455720907538425TruSeq Adapter, Index 11 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC6650.33798894039196553No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC5210.26480035781084815No Hit
TCATATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG4820.24497845002846222No Hit
CTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTC2980.15145970561925673No Hit
AATATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT2580.13112954379116856No Hit
CATATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT2380.1209644628771245No Hit
CAAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT2350.11943970074001788No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCAACATCTCGTATGCCGT2110.10724160364316501Illumina PCR Primer Index 6 (95% over 21bp)
TCTAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG2080.10571684150605838No Hit
AAAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT2040.10368382532324957No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGTT150.002219692370.0195832
GCCATAG207.9113204E-570.001781
TTCCGTC150.002224173669.9839945
CGTCTAC150.002224173669.9839948
ATTCCGT150.002224173669.9839944
TCGTATT150.002224173669.9839940
TCCGTCT150.002224173669.9839946
ACGTATG401.3460522E-1069.9839940
CACGTAT401.3460522E-1069.9839939
CTCTCGT150.002224173669.9839937
AGGGGGG44600.064.4135270
ATTATAC9350.063.2636381
ATATCCG401.0630174E-861.23599210
TCGACCA750.060.6990729
TCACGTA601.8189894E-1258.31999638
TCGAATG553.6379788E-1157.2596340
CTCGACC800.056.90537628
GCCCAGT252.3828557E-456.01566319
CCATAGA252.385852E-456.0014232
TCGCACA1200.055.44626629
Produced by FastQC (version 0.11.5)