FastQCFastQC Report
Wed 25 May 2016
SRR1780105_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780105_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233274
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5255422.52887162735667No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG172877.410598695096753No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT15270.654595025592222No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG12310.527705616571071No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC11390.4882670164699023No Hit
TCTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG5820.2494920136834795No Hit
CTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG2660.11402899594468309No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGCC150.002217765270.0390332
GGTACAA150.00222343269.9939962
TAAGCCG150.00222343269.9939965
GCTCTAG252.388086E-455.9951971
AAAAAGG17800.055.65322570
GATCTCG26350.053.25919341
GCCGTAT26100.053.09889654
TCGCCGT26250.053.0621252
ATCTCGG26450.053.05783542
CTCGGTG26450.053.05783544
CGGTGGT26450.053.05783546
TGGTCGC26450.053.05783549
AGATCTC26500.052.9577240
CGTATCA26250.052.928856
GTATCAT26450.052.79320557
TAGATCT26450.052.79320539
TCGGTGG26650.052.79097445
GTAGATC26550.052.72617738
CCGTATC26300.052.695155
GTCGCCG26500.052.69359651