FastQCFastQC Report
Wed 25 May 2016
SRR1780105_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780105_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233274
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2581111.0646707305572No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT2370310.161012371717378No Hit
ATTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT79813.4212985587763747No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG34701.4875211125114671No Hit
CTAATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT29131.2487461097250443No Hit
ATAATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT18130.7771976302545505No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC17780.762193814998671No Hit
ATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTCTT7250.3107933160146437RNA PCR Primer, Index 30 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC7000.30007630511758704No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC5870.25163541586289084No Hit
TCATATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG4450.1907627939676089No Hit
CTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTC3240.13889246122585458No Hit
AATATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT3020.1294614916364447No Hit
CATATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT2790.11960184161115256No Hit
CAAATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT2490.10674142853468453No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC150.002223107369.99657453
TGTACGT150.002223107369.9965743
GTACGTA150.002223107369.9965744
TACGTAG150.002223107369.9965745
CGGACCT150.002223107369.99657454
GCCGTAT207.917317E-569.9965746
CGCCCGT207.917317E-569.9965710
TGCCGTA207.917317E-569.9965745
AGGGGGG44400.065.1882570
ATTATAC10100.059.947561
TAATACA8150.059.6903272
CCGACTT601.8189894E-1258.3304847
CGACTTC601.8189894E-1258.3304848
AATACAC9450.057.034243
CGTCTAC252.387653E-455.99725348
AAGGGGG51700.055.57754569
GCCGTCT51400.054.74439646
TATGCCG52500.054.59732443
CCGTCTT51400.054.47203447
ACTCCGA452.6921043E-854.4417811
Produced by FastQC (version 0.11.5)