FastQCFastQC Report
Wed 25 May 2016
SRR1780104_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780104_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167002
Sequences flagged as poor quality0
Sequence length76
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6840440.9599885031317No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG2408614.422581765487838No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT21281.274236236691776No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC15310.916755487958228No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG15280.9149591022862No Hit
TCTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG7420.4443060562148956No Hit
CTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG3620.21676387109136416No Hit
CTTATACACATCTGACGCTGCCGACGAATGATGGAGTGTAGATCTCGGTG2360.14131567286619323No Hit
CTTTACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG2350.14071687764218394No Hit
CTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT2200.13173494928204452No Hit
TCTTAATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG1910.114369887785775No Hit
CTTAATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT1850.11077711644171925No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACAT150.002221642369.99940539
CGATTTA150.002221642369.99940525
TGTGGCG207.909158E-569.999411
CGACCAT207.909158E-569.999422
AAAAAGG24000.058.35030770
GTGTGCG308.349323E-658.3328362
GTTGTGC308.349323E-658.3328361
CTTTATA1200.055.4161952
CTATACA700.054.9995271
TCTTTAT1150.054.782141
AGCCGAC452.6851922E-854.44397419
TTGTGCG452.6851922E-854.4439742
TTTATAC1500.053.666213
TCTGCGC200.00694192552.49954646
CGGGTGT200.00694192552.4995467
TGTCAAC200.00694192552.49954610
GTTTCTT200.00694192552.4995469
ATGGCTC200.00694192552.4995467
CATTGAT200.00694192552.49954626
ACGATTT200.00694192552.49954624
Produced by FastQC (version 0.11.5)