FastQCFastQC Report
Wed 25 May 2016
SRR1780104_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780104_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167002
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3555921.29255937054646No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT3041518.212356738242654No Hit
ATTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT90355.410114848923965No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG44822.6838001940096525No Hit
CTAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT42652.553861630399636No Hit
ATAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT21711.2999844313241757No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC19161.147291649201806No Hit
ATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTCTT10230.612567514161507TruSeq Adapter, Index 6 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC8170.4892156980155926No Hit
ATCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC5980.35807954395755737No Hit
TCATATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG5680.3401156872372786No Hit
CTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC3960.23712290870768013No Hit
CAAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT3510.21017712362726196No Hit
CATATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT3260.19520724302702963No Hit
AATATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT2920.17484820541071366No Hit
AAAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT2580.15448916779439767No Hit
CTTATACACATCTCCGAGCCCACGAGACACAACAACATCTCGTATGCCGT2510.15029760122633262No Hit
TCTAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG2300.13772290152213745No Hit
TTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC1970.11796265912983078No Hit
CTTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG1850.11077711644171925No Hit
TATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTCT1800.1077831403216728No Hit
CTTAAACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT1750.10478916420162633No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTGGA150.002217911270.0290628
CATTTGC150.002220548770.0080933
CGAAAGC207.916028E-569.9871341
TGACGAC207.916028E-569.9871345
CCGTCAA207.916028E-569.9871347
ACGTAAG207.916028E-569.9871340
GACGACT207.916028E-569.9871346
TGGTTAA150.002223188369.9871237
CAAATCG150.002223188369.9871236
CTACAGC150.002223188369.9871251
ACGTATT150.002223188369.9871247
ACGACTA150.002223188369.9871247
ATTATAC12400.066.0758061
AGGGGGG56550.065.2223170
ATCTTAT700.065.026981
ATAATAC4400.063.6627771
AATATAC900.062.2480551
TAATACA10650.061.809692
AATACAC12850.061.3094863
CTAATAC6300.060.5806961
Produced by FastQC (version 0.11.5)