FastQCFastQC Report
Wed 25 May 2016
SRR1780103_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780103_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266577
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7123126.720609805046948No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG259949.751028783428428No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT24140.9055544926981698No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC17230.6463423326093399No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG16310.6118307280823178No Hit
TCTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG7980.29935065665830135No Hit
CTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG3700.1387966703804154No Hit
CTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT2840.10653582267037291No Hit
CTTTACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG2700.10128405676408693No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTC150.002223024470.0000160
CGACGTA150.002223024470.0000153
CCGAGTT150.002223024470.0000159
TAGTCGA150.002223024470.000012
GATCCTA352.9672447E-760.01
AAAAAGG24400.056.2295170
CCGAATC252.3876948E-456.00000455
AAGTATC200.0069462152.58
TCGTTGT200.0069462152.534
GACGTAT200.0069462152.554
TAACGGC200.0069462152.523
GGCGGTA200.0069462152.541
CATGTTC200.0069462152.534
TCGACGT200.0069462152.552
TGGTCGA200.0069462152.549
ATCTCGG37800.050.7407442
GATCTCG37800.050.7407441
CTCGGTG37900.050.69920744
TCTCGGT38000.050.65789843
TCGGTGG38100.050.616845