FastQCFastQC Report
Wed 25 May 2016
SRR1780103_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780103_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266577
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3817414.320065121897239No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT3468913.01275053736819No Hit
ATTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT79993.000633963170116No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG49391.852747986510464No Hit
CTAATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT48641.8246135262982177No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC23820.8935504563409447No Hit
ATAATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT20480.7682583268624075No Hit
ATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTCTT12740.47791069747202497Illumina PCR Primer Index 10 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC9940.37287537934630516No Hit
TCATATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG6920.25958728622499316No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC5520.20706962716213326No Hit
CTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTC5170.1939402123964183No Hit
CATATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT4110.1541768419631101No Hit
CAAATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT3940.14779969764833426No Hit
AATATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT3190.11966523743608788No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT252.8366758E-669.99306546
TGCCGTA252.8366758E-669.99306545
ACGATCC150.002223899469.9930658
GAGCGCC150.002223899469.9930654
AACGATC150.002223899469.9930657
AGGGGGG59800.064.08227570
TATGACG1450.062.752443
ATTATAC9600.061.608471
ATAATAC4500.059.882951
CGTATGA1700.059.69996341
TAATACA11150.059.00762
ACGTATG308.367435E-658.32754540
TAACTCG850.057.64134236
GTATGAC1650.057.2670542
AATACAC13100.056.9027523
ATGACGT1550.056.44601444
AATCACG252.388865E-455.9944536
AATCTCG950.055.25767536
CGTCTAC700.054.99454548
CGAATGC452.6951966E-854.4390541
Produced by FastQC (version 0.11.5)