FastQCFastQC Report
Wed 25 May 2016
SRR1780102_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780102_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101737
Sequences flagged as poor quality0
Sequence length76
%GC82

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5795756.96747495994574No Hit
CTTATACACATCTGACGCTGCCGACGACGATACACGTGTAGATCTCGGTG9110.8954461012217777No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3600.35385356360026343No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2060.20248287250459518No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1040.10222436281785388No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAA150.002219061670.061
GTAGGGC150.002219061670.038
TGTGCGG150.002219061670.03
TGTGGCG308.325524E-658.33333211
GCGGCTG252.380653E-455.9999968
CTTGTAC200.00693392152.51
GACAGAA200.00693392152.55
TAGGTGA200.00693392152.519
GGGTATA200.00693392152.51
TGCGGGT200.00693392152.55
GCTGAGC200.00693392152.521
CCGTATC1200.052.555
GGGGTGC200.00693392152.51
CGGGCGC407.4164745E-752.512
GAAAAAC200.00693392152.525
TATACCC200.00693392152.53
GCGTCGG200.00693392152.58
AGGTGTA200.00693392152.534
CTTACTA200.00693392152.53
ATCTGGC200.00693392152.510
Produced by FastQC (version 0.11.5)