FastQCFastQC Report
Wed 25 May 2016
SRR1780102_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780102_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101737
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT2954029.035650746532728No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG58735.772727719512075No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC23752.3344505931961823No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15931.5658020189311654No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT13881.364302072992127No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT13451.3220362306732065No Hit
ATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCTT9160.9003607340495592RNA PCR Primer, Index 41 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACGT9100.8944631746562215No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTAAGCCGT8720.8571119651650826No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC8540.8394192869850693No Hit
ATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCAT5480.5386437579248454No Hit
CTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC4870.4786852374259119No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGA3960.38923891996028975No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCG3660.3597511229936011No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT3360.33026332602691255No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCCT3170.3115877212813431No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAAAGACATCTCGTATGCCGT3160.3106047947157868No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCG2730.26833895239686645No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATTCCGT2640.2594926133068598No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGAATGCCGT2490.24474871482351554No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCAAGTATGCCGT2280.2241072569468335No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGCCATCTCGTATGCCGT2010.19756823967681372No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCAGT1870.18380726775902573No Hit
CCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG1830.17987556149680056No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGAAATCTCGTATGCCGT1800.17692678180013172No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG1730.1700462958412377No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG1730.1700462958412377No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATATCGTATGCCGT1720.1690633692756814No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC1710.16808044271012512No Hit
TTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC1680.16513166301345628No Hit
TATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCT1600.15726825048900597RNA PCR Primer, Index 41 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCC1480.1454731317023305No Hit
CTTTACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC1440.14154142544010537No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACG1330.1307292332189862No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATTCCG1330.1307292332189862No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTAAGCCGT1320.1297463066534299No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCCTATGCCGT1280.12581460039120476No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCC1240.12188289412897962No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGACGT1120.11008777534230417No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCAG1110.10910484877674788No Hit
CTTATACACATCTCCGAGACCACGAGACAATAAGACATCTCGTATGCCGT1070.10517314251452274No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTAAGCCGT1050.10320728938341016No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCAA252.822404E-670.053
AGCTTTA150.002219061670.055
CTCTTCT207.893972E-570.049
CACGAAT252.822404E-670.039
AGAACAC207.893972E-570.017
CCGTCAA150.002219061670.047
TATGACC252.822404E-670.043
GCCGAAA353.6689016E-970.046
ACAGCAT252.822404E-670.053
CGAGAAC207.893972E-570.015
AACTCGT301.0149233E-770.037
CCGAAAT301.0149233E-770.047
CAACTCG353.6689016E-970.036
GAACACG207.893972E-570.018
TACAGCA207.893972E-570.052
CGTCAAC150.002219061670.048
ACGACAC150.002219061670.022
ACGAATG207.893972E-570.040
TCTTTAT1650.067.8787841
GACGTCA750.065.33333646
Produced by FastQC (version 0.11.5)