FastQCFastQC Report
Wed 25 May 2016
SRR1780101_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780101_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282637
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3706813.115055707497604No Hit
CTTATACACATCTGACGCTGCCGACGACGATACACGTGTAGATCTCGGTG4240.1500157445769662No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCGA252.3881513E-455.99901212
GCCGTAT701.9197614E-839.99929454
AACGCTG459.185254E-538.888270
TCGGTGG901.03682396E-1038.888245
GCATAAG501.7074053E-434.9993821
CGTATCA1003.237801E-1034.99938256
CCGTATC806.223672E-834.99938255
TCGGACT400.00241656734.99938213
GGACCTT501.7074053E-434.9993821
CGGTGGT904.485628E-934.9993846
GTAATCC552.9880967E-431.817624
GCTATAA1001.2534656E-831.4994431
TCGCCGT901.751032E-731.11055852
TGGTCGC853.945661E-628.8230249
AGATCTC1253.5361154E-927.99950640
GTATCAT1305.3751137E-926.92260257
TAGATCT1207.3698175E-826.24953739
GATCTCG1207.3698175E-826.24953741
GTGGTCG1207.3698175E-826.24953748
ATCTCGG1251.09426765E-725.19955642
Produced by FastQC (version 0.11.5)