FastQCFastQC Report
Wed 25 May 2016
SRR1780101_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780101_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282637
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT212157.506094389623439No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG46651.6505270010649702No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC17190.6082006248297286No Hit
ATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTCTT12270.4341257514055131Illumina PCR Primer Index 5 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7490.2650042280380842No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC7460.2639427958830585No Hit
ATTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT4170.1475390695485729No Hit
CTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTC3750.13267901937821305No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCATTGATCTCGTATGCCGT3070.10861989053096374No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCATTGCTCTCGTATGCCGT2960.10472797262920285No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGTG150.002225215369.983727
TGGTACG150.002225215369.9837210
TCGAATG453.8380676E-1062.21876540
AGGGGGG26800.061.25743570
ACGTATG352.9690273E-759.9966640
CGAACCG200.00694330452.50637460
GCGTAAA200.00694330452.5063741
ACGTTAC200.006948166552.4970856
TAACGGT200.006948166552.4970846
CCCGTGT200.006948166552.4970853
TCATCGT200.006948166552.4970838
GAACCGG200.006948166552.4970848
TATCCGG200.006948166552.4970840
CCAACGT200.006953031352.48779312
CCGACTT608.367351E-1152.4877947
CCGTCTT37050.049.20583347
GCCGTCT37500.049.1839346
TATGCCG38300.049.16175543
CGTCTTC37400.049.1282848
CGTATGC38750.048.86179741
Produced by FastQC (version 0.11.5)