FastQCFastQC Report
Wed 25 May 2016
SRR1780100_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780100_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335773
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5128815.274605164798839No Hit
CTTATACACATCTGACGCTGCCGACGACGATACACGTGTAGATCTCGGTG40191.1969395990743747No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCGA150.002223566469.99971
TCGCCGT6450.058.06176452
GCCGTAT6600.057.8027854
CGGTGGT7050.056.0990546
CTCGGTG7000.055.9997644
TCTCGGT7000.055.9997643
TGGTCGC6950.055.89904449
AGATCTC7100.055.7039940
GATCTCG7050.055.60259641
GTCGCCG7050.055.10614451
TCGGTGG7250.055.0342545
GTAGATC7000.054.99976338
ATCTCGG7150.054.82493642
TAGATCT7200.054.44421439
CGTATCA7150.054.3354356
CGCCGTA7100.053.23921253
CCGTATC7200.052.98588655
GTATCAT7200.052.98588657
TACCGAC200.006943797752.50759569
AGCAACG200.006947889552.499785
Produced by FastQC (version 0.11.5)