FastQCFastQC Report
Wed 25 May 2016
SRR1780100_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780100_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335773
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT319159.504933392500291No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG67101.9983739014155395No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60561.8035994555845765No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC24200.7207250136252766No Hit
ATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTCTT16030.4774058664633547TruSeq Adapter, Index 8 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC10920.32521971689206697No Hit
ATTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT5410.16112075717821264No Hit
CTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTC5320.1584403748961352No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT4400.1310409115682321No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGACGT3540.105428369761714No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACCG150.002223941569.9967312
ACCCGTT150.002223941569.9967359
AGGGGGG39650.060.99336270
CGAATGC601.8189894E-1258.33060541
CGTCTAC509.786163E-1055.9973848
GCCGACT509.786163E-1055.9973846
AATCACG452.6968337E-854.441936
CGTGGGA200.00694496652.5053632
CGAAATC200.00694496652.50536361
CGAGTGG200.006949058352.49754736
TGTTCTA200.006949058352.49754710
ACGTATG407.470753E-752.49754740
TGACGTC2050.051.21711345
CCGACTT552.2791937E-950.90670847
CGTATGA2050.049.50987641
GTATGAC2050.047.8026442
CGTCTTC56200.047.7023948
GCCGTCT55150.047.59523446
GTCTTCT55950.047.29001649
CCGTCTT55000.047.27960647
Produced by FastQC (version 0.11.5)