FastQCFastQC Report
Wed 25 May 2016
SRR1780099_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780099_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919632
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG337733.6724472397654715No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT1200.046.665854
CGCCGTA1300.045.7683853
GTCGCCG1350.044.07325451
TGGACCG404.594591E-543.7491875
TCGCCGT1400.042.49920752
TGGTCGC1400.039.99925649
TCGGTGG1500.039.66592845
CCGTATC1550.036.12835755
CGGTGGT1950.034.1019346
CGTATCA1650.033.93876356
GTGGTCG1800.033.0549448
CTCGGTG1800.031.11222344
TACTCGC450.004308411431.11053310
GCCGCTA500.00720837328.00100169
TTAACGG500.00720837328.00100135
TCTCGGT2100.026.6676243
GGTCGCC2350.025.31867650
CGATACA14700.023.5722770
GCCGGCG1202.16851E-623.332920
GGTGGTC2900.021.72373247
Produced by FastQC (version 0.11.5)