FastQCFastQC Report
Wed 25 May 2016
SRR1780099_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780099_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919632
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT155191.6875228352210452No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG47480.5162934739113036No Hit
ATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCTT12690.1379899786001357TruSeq Adapter, Index 18 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC11380.12374515023400666No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG22500.053.1950470
CGCAAAT305.87676E-446.6699334
CGTATGC32800.041.93206841
GCCGTCT30950.041.8377846
TATGCCG32150.041.80014443
TCTCGTA33950.041.74868838
TCGTATG34300.041.73080440
ATGCCGT32300.041.71437544
CGTCTTC30600.041.63010448
TGCCGTC31800.041.59989545
CCGTCTT31050.041.5903347
ATCTCGT34200.041.4435137
CTCGTAT33900.041.39732439
CATCTCG35200.040.4649836
GTATGCC33600.040.20458642
GCGCATC35450.039.4971533
CGCATCT35850.039.05433734
TATGACG1450.038.6170943
CTGCTTG33100.037.74572854
GGCGCAT37300.037.63611632
Produced by FastQC (version 0.11.5)