FastQCFastQC Report
Wed 25 May 2016
SRR1780098_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780098_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200326
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3966819.80172319119835No Hit
CTTATACACATCTGACGCTGCCGACGACGATACACGTGTAGATCTCGGTG6100.3045036590357717No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCA150.002223661969.9885212
TGCGCGT252.3881922E-455.990815
CGTATCA1100.054.0955456
CGTTGTT200.00694134852.50449835
ATTCCTC200.00694134852.50449829
AACGACG200.00694134852.50449870
CCGTGCA200.006948206652.4913910
TGCGGGC200.006948206652.4913919
CCAAACG200.006948206652.4913915
TCTCGGT1350.051.8562943
GTCGCCG1300.051.15822651
TCGCCGT1100.050.9134552
CGCCGTA1100.050.9134553
CGGTGGT1450.050.69399346
TCGGTGG1550.049.6816745
CCGTATC1200.049.58757855
GCCGTAT1200.049.58757854
TGGTCGC1450.048.27999549
CTCCGTC305.872465E-446.659019
GGACTTA451.6806753E-646.6590081
Produced by FastQC (version 0.11.5)