FastQCFastQC Report
Wed 25 May 2016
SRR1780098_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780098_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200326
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT197499.858430757864681No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCG39681.9807713427113804No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC15360.7667501971785988No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCGT14550.7263161047492587No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10730.5356269281071853No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGACGT9180.458253047532522No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTAAGCCGT7150.3569182232960275No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC6210.30999470862494133No Hit
ATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTCTT5950.2970158641414494RNA PCR Primer, Index 26 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCG3630.18120463644259857No Hit
ATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTCAT3200.15973962441220813No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGA3060.152751015844174No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCCT2760.1377754260555295No Hit
CTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTC2710.13527949442408874No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATTCCGT2670.13328274911893612No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT2620.13078681748749538No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGA207.9052596E-570.0132428
ACGCCTT150.0022205570.0132330
GACGTAC150.0022205570.0132326
ACGTACG150.0022205570.0132327
ACGAGTA252.8292507E-670.0132331
TGCGTGG150.0022205570.0132329
TGACGAA150.002222750669.9957545
AGGGGGG23300.061.43404470
ATCGTAT401.0617441E-861.24628439
TAAGCCG1350.059.6260243
GCCGTAA1500.058.329846
CGAAATA252.386972E-455.99660548
ATATCGT509.767973E-1055.99660537
AACTCGT252.386972E-455.99660537
TATCGTA509.767973E-1055.99660538
TGCCGAC509.767973E-1055.99660545
AGCCGTC1200.055.41330345
TTCGAGA900.054.45473530
TAAGACG452.6899215E-854.44114343
CGAGAAT653.6379788E-1253.8563332