FastQCFastQC Report
Wed 25 May 2016
SRR1780097_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780097_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54431
Sequences flagged as poor quality0
Sequence length76
%GC93

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4166776.5501276845915No Hit
CTTATACACATCTGACGCTGCCGACGACGATACACGTGTAGATCTCGGTG5300.9737098344693282No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1130.20760228546232842No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG860.1579981995554004No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG640.11758005548308868No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG590.10839411364847239No Hit
GGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGG560.1028825485477026No Hit
GGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG550.10104536018077934No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG550.10104536018077934No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG550.10104536018077934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTGT150.002207036870.0515233
TGGGCGC150.002215111269.987143
GGGTGTA150.002215111269.9871434
GGTGTAG150.002215111269.9871435
CTATTAG150.002215111269.987147
ACACGTC352.9269177E-759.988976
CACGTCT308.282739E-658.3226137
GGCGCTG608.0035534E-1152.5386414
TGGCGCT552.222805E-950.89973413
GCGCTGC352.052136E-550.036815
CTGCCGG352.052136E-550.036818
ACGTCTG352.0633533E-549.990818
GTATCAT950.047.8859357
CATTAAA950.047.8859361
TATCATT950.047.8859358
ATCATTA950.047.8859359
TCATTAA950.047.8859360
GCGCTGG305.808728E-446.7010115
CTGGCGC604.800313E-946.65809212
GTCTGGC451.6582435E-646.6580910