##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780097_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 54431 Sequences flagged as poor quality 0 Sequence length 76 %GC 93 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.788025206224393 32.0 27.0 32.0 13.0 32.0 2 28.483492862523196 32.0 32.0 32.0 13.0 32.0 3 28.397935000275577 32.0 27.0 32.0 13.0 32.0 4 28.006411787400562 32.0 27.0 32.0 13.0 32.0 5 28.683838253936177 32.0 32.0 32.0 13.0 32.0 6 32.644412191582006 37.0 32.0 37.0 13.0 37.0 7 32.03303264683728 37.0 32.0 37.0 13.0 37.0 8 32.65681321305873 37.0 32.0 37.0 13.0 37.0 9 32.63851481692418 37.0 32.0 37.0 13.0 37.0 10-11 32.57536146681119 37.0 32.0 37.0 13.0 37.0 12-13 32.20505777957414 37.0 32.0 37.0 13.0 37.0 14-15 33.24033179621907 37.0 34.5 37.0 17.5 37.0 16-17 33.237318807297314 37.0 34.5 37.0 17.5 37.0 18-19 32.532802998291416 37.0 32.0 37.0 13.0 37.0 20-21 33.14150943396227 37.0 34.5 37.0 17.5 37.0 22-23 33.18700740386912 37.0 34.5 37.0 17.5 37.0 24-25 32.864057246789514 37.0 34.5 37.0 13.0 37.0 26-27 33.33645349157649 37.0 34.5 37.0 17.5 37.0 28-29 33.131882566919586 37.0 37.0 37.0 13.0 37.0 30-31 33.090187576932266 37.0 34.5 37.0 13.0 37.0 32-33 33.16889272657126 37.0 37.0 37.0 13.0 37.0 34-35 33.27008506182139 37.0 37.0 37.0 13.0 37.0 36-37 33.93782954566332 37.0 37.0 37.0 24.5 37.0 38-39 34.0665797064173 37.0 37.0 37.0 24.5 37.0 40-41 33.51966710146792 37.0 37.0 37.0 17.5 37.0 42-43 33.46895151659899 37.0 37.0 37.0 22.0 37.0 44-45 33.21544707978909 37.0 37.0 37.0 13.0 37.0 46-47 33.41043706711249 37.0 34.5 37.0 17.5 37.0 48-49 34.06928037331667 37.0 37.0 37.0 24.5 37.0 50-51 34.38389888115229 37.0 37.0 37.0 27.0 37.0 52-53 33.486560967095954 37.0 37.0 37.0 17.5 37.0 54-55 33.67219047968988 37.0 37.0 37.0 20.0 37.0 56-57 33.52533482757987 37.0 37.0 37.0 17.5 37.0 58-59 33.24110341533317 37.0 37.0 37.0 13.0 37.0 60-61 32.98914221675149 37.0 34.5 37.0 13.0 37.0 62-63 32.67111572449523 37.0 34.5 37.0 13.0 37.0 64-65 32.48413587845162 37.0 32.0 37.0 13.0 37.0 66-67 32.62875016075398 37.0 32.0 37.0 13.0 37.0 68-69 32.696634270911794 37.0 32.0 37.0 13.0 37.0 70-71 32.68892726571255 37.0 32.0 37.0 13.0 37.0 72-73 33.12961363928643 37.0 34.5 37.0 13.0 37.0 74-75 33.57436938509305 37.0 37.0 37.0 17.5 37.0 76 34.52686887986625 37.0 37.0 37.0 27.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 11101 1 -2.0149306275611636 11101 2 0.7954868590182116 11101 3 0.6527748186202231 11101 4 0.6670938389198149 11101 5 2.0255311999193886 11101 6 0.6656008824865154 11101 7 -1.6622829400642196 11101 8 1.0210992807667694 11101 9 -1.1438518695135222 11101 10-11 1.8191651908928037 11101 12-13 1.0340063793627507 11101 14-15 1.3851940730799086 11101 16-17 0.8642125360082176 11101 18-19 -1.2747835710199027 11101 20-21 1.0776728321473996 11101 22-23 0.35343666460429546 11101 24-25 0.08169463122791143 11101 26-27 2.0300821051261337 11101 28-29 0.6633369058093024 11101 30-31 2.2022309323408393 11101 32-33 0.6044899061166831 11101 34-35 -0.24471622483373068 11101 36-37 1.1821897624308733 11101 38-39 0.9297221422834809 11101 40-41 0.273368734851438 11101 42-43 -0.428563444008212 11101 44-45 0.3538249566617466 11101 46-47 -0.6281360194377186 11101 48-49 -0.9350061023740324 11101 50-51 0.6432037170123834 11101 52-53 0.049856431390196576 11101 54-55 0.14754119310671143 11101 56-57 0.6302611266097813 11101 58-59 -0.21225946896220904 11101 60-61 -0.9953635049079352 11101 62-63 -0.6553027804070339 11101 64-65 0.604107573603514 11101 66-67 0.7047202360647447 11101 68-69 0.1707464479946168 11101 70-71 1.294367226057517 11101 72-73 -0.5223145030963678 11101 74-75 -0.40667466988837475 11101 76 0.6617431395538844 11102 1 0.9107988154096596 11102 2 1.7583515805036214 11102 3 1.4564883464716836 11102 4 1.5503829635882518 11102 5 1.5242049399724387 11102 6 0.9162640124599903 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-0.3616847563185317 21612 60-61 -0.2178593998176268 21612 62-63 0.08402398478180118 21612 64-65 0.41198934700253176 21612 66-67 -0.06046859808959937 21612 68-69 -0.33951627778535 21612 70-71 0.2635790487176166 21612 72-73 -0.6774869168894782 21612 74-75 -0.5712887913990485 21612 76 0.6321864893075855 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 3.0 11 8.0 12 32.0 13 75.0 14 139.0 15 234.0 16 374.0 17 527.0 18 651.0 19 674.0 20 625.0 21 632.0 22 679.0 23 726.0 24 777.0 25 901.0 26 974.0 27 1059.0 28 1186.0 29 1342.0 30 1515.0 31 1813.0 32 2240.0 33 2968.0 34 4211.0 35 7228.0 36 22838.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 89.52435193180357 2.2321838658117614 1.965791552607889 6.277672649776782 2 87.09926328746486 2.7668056805864305 7.106244603259173 3.0276864286895333 3 86.99270636218331 2.5536918300233324 7.730888648013081 2.7227131597802723 4 88.21422009921 4.708800293955539 3.984934778614734 3.092044828219732 5 87.26277305212103 3.8709558891073104 7.052966140618398 1.8133049181532583 6 89.64725335293036 6.079367995590667 2.336946536836303 1.9364321146426604 7 88.28587176189602 3.729560903913283 2.3553187580378467 5.629248576152857 8 89.57211882934357 5.794492109275964 2.2964854586540757 2.3369036027263874 9 87.53835130715953 2.5169480626848673 1.9070015248663448 8.037699105289265 10-11 89.14864691076775 3.9113740331796216 4.346787676140434 2.5931913799121826 12-13 88.36487231306265 2.976299834650009 4.343193092044828 4.315634760242514 14-15 90.67893919769247 3.676247692010913 2.6363895242557023 3.0084235860409145 16-17 90.02590435597362 3.028605022872995 2.482041483713325 4.463449137440062 18-19 87.86445224228841 2.870606823317595 4.277893112380812 4.98704782201319 20-21 90.31674291278546 2.6180896213415643 2.90562016572048 4.159547300152492 22-23 90.11307998273027 2.598726816766335 2.6676220133932262 4.620571187110168 24-25 89.30920448282197 3.865515340804703 2.553738747014514 4.271541429358809 26-27 90.74866801396288 3.9537020025721112 2.5739481903362114 2.7236817931287893 28-29 90.24894359728091 3.042439830975565 3.105823994120889 3.602792577622634 30-31 89.42852680991007 3.9877272434984707 3.786549573301243 2.7971963732902196 32-33 89.57724742324864 3.9997060390600603 2.9001083980966027 3.5229381395947015 34-35 89.71523057137608 4.0483189417600585 3.0286606650744075 3.2077898217894543 36-37 90.67334190703656 2.735623736909792 4.0795517178026826 2.5114826382509645 38-39 90.87092714562607 2.5996454193880267 4.288955640679398 2.240471794306501 40-41 90.78466315151292 3.7469456743399903 3.0377909647076113 2.430600209439474 42-43 89.8190336211648 3.584420356421091 4.051993386000367 2.5445526364137425 44-45 89.42954253169208 2.615285688039684 4.293588094800661 3.661583685467573 46-47 90.35090942494948 2.518831526731582 3.1517545471247472 3.9785045011941946 48-49 90.88019694659295 2.5261340045194833 4.060186290900406 2.5334827579871764 50-51 91.84464592461947 2.4737968602162392 3.1590744159983837 2.522482799165909 52-53 89.58497914791204 2.7420036376329664 4.026198306112326 3.6468189083426723 54-55 90.52286380922637 2.651981407653727 2.967059212581066 3.8580955705388478 56-57 90.60645587992137 2.6069702926641067 2.8733626058679795 3.9132112215465455 58-59 89.61887177226004 3.82597991934669 3.8140380852646953 2.741110223128577 60-61 89.35441200786317 2.909187779022983 4.0023148573423235 3.7340853557715272 62-63 89.5316045231993 3.882014679269895 3.7736195699102524 2.812761227620544 64-65 89.15304060260885 4.302774205401433 3.885724784126401 2.658460407863311 66-67 89.15038443519718 5.5474412323972775 2.580354764332497 2.7218195680730473 68-69 89.0806709411916 5.776120225606732 2.559203395124102 2.584005438077566 70-71 89.41136484723778 5.472065550880932 2.3442523561940805 2.7723172456872005 72-73 89.72827984053205 5.163417905237824 2.3470081387444655 2.761294115485661 74-75 90.66708309602983 4.339438922672741 2.4021237897521632 2.591354191545259 76 92.20636057983795 2.833048558672766 2.4086424516342393 2.5519484098550405 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 1.0 15 2.0 16 3.5 17 5.0 18 6.0 19 13.0 20 14.5 21 10.0 22 17.5 23 18.5 24 20.5 25 29.0 26 42.5 27 64.0 28 71.0 29 70.0 30 76.0 31 75.5 32 84.0 33 99.0 34 133.5 35 155.5 36 157.5 37 172.0 38 164.5 39 204.0 40 256.0 41 271.5 42 282.0 43 342.0 44 571.0 45 617.0 46 494.0 47 384.5 48 271.5 49 263.5 50 259.0 51 232.0 52 182.5 53 180.5 54 201.0 55 183.5 56 175.0 57 160.0 58 136.0 59 140.0 60 147.0 61 125.5 62 101.0 63 99.5 64 85.0 65 84.5 66 94.5 67 92.0 68 79.5 69 72.5 70 75.0 71 72.0 72 67.5 73 59.0 74 55.5 75 56.0 76 58.5 77 62.5 78 75.0 79 86.0 80 85.0 81 85.0 82 85.0 83 84.0 84 85.0 85 92.0 86 108.0 87 118.0 88 124.5 89 165.0 90 201.5 91 224.0 92 244.0 93 279.5 94 334.5 95 436.0 96 518.0 97 658.5 98 1272.5 99 22135.5 100 42525.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0018371883669232607 5 0.0 6 0.0018371883669232607 7 0.0018371883669232607 8 0.0 9 0.0 10-11 0.0 12-13 0.0018371883669232607 14-15 9.185941834616303E-4 16-17 0.0 18-19 0.0 20-21 0.0036743767338465213 22-23 9.185941834616303E-4 24-25 0.0018371883669232607 26-27 0.0018371883669232607 28-29 0.0018371883669232607 30-31 0.002755782550384891 32-33 0.0036743767338465213 34-35 0.0018371883669232607 36-37 0.0018371883669232607 38-39 9.185941834616303E-4 40-41 0.0 42-43 0.0018371883669232607 44-45 0.0018371883669232607 46-47 0.0018371883669232607 48-49 0.0 50-51 9.185941834616303E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 9.185941834616303E-4 60-61 0.0 62-63 9.185941834616303E-4 64-65 0.0018371883669232607 66-67 9.185941834616303E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0036743767338465213 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 54431.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.930829857985339 #Duplication Level Percentage of deduplicated Percentage of total 1 85.97268364710226 12.83643511969282 2 5.487879906484558 1.6387720232955487 3 3.2853451458102625 1.471587881905532 4 1.845699520118125 1.1023130201539564 5 0.9720684139288791 0.7256894049346879 6 0.6152331733727082 0.5511565100769782 7 0.49218653869816664 0.514412742738513 8 0.15996062507690414 0.19106759016001912 9 0.2830072597514458 0.380297991953115 >10 0.7382798080472499 1.8702577575278796 >50 0.11074197120708748 0.9865701530377909 >100 0.012304663467454166 0.20760228546232842 >500 0.012304663467454166 0.9737098344693282 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.012304663467454166 76.5501276845915 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 41667 76.5501276845915 No Hit CTTATACACATCTGACGCTGCCGACGACGATACACGTGTAGATCTCGGTG 530 0.9737098344693282 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 113 0.20760228546232842 No Hit GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 86 0.1579981995554004 No Hit GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 64 0.11758005548308868 No Hit GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 59 0.10839411364847239 No Hit GGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGG 56 0.1028825485477026 No Hit GGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 55 0.10104536018077934 No Hit GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 55 0.10104536018077934 No Hit GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 55 0.10104536018077934 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0018371883669232607 0.0 2 0.0 0.0 0.0 0.0018371883669232607 0.0 3 0.0 0.0 0.0 0.0018371883669232607 0.0 4 0.0 0.0 0.0 0.012860318568462824 0.0 5 0.0 0.0 0.0 0.042255332439234995 0.0 6 0.0 0.0 0.0 0.042255332439234995 0.0 7 0.0 0.0 0.0 0.042255332439234995 0.0 8 0.0 0.0 0.0 0.042255332439234995 0.0 9 0.0 0.0 0.0 0.042255332439234995 0.0 10 0.0 0.0 0.0 0.042255332439234995 0.0 11 0.0 0.0 0.0 0.045929709173081514 0.0 12 0.0 0.0 0.0 0.045929709173081514 0.0 13 0.0 0.0 0.0 0.045929709173081514 0.0 14 0.0 0.0 0.0 0.045929709173081514 0.0 15 0.0 0.0 0.0 0.05695283937462108 0.0 16 0.0 0.0 0.0 0.05695283937462108 0.0 17 0.0 0.0 0.0 0.05695283937462108 0.0 18 0.0 0.0 0.0 0.06797596957616064 0.0 19 0.0 0.0 0.0 0.11023130201539563 0.0 20 0.0 0.0 0.0 0.1212544322169352 0.0 21 0.0 0.0 0.0 0.15064944608770736 0.0 22 0.0 0.0 0.0 0.1635097646561702 0.0 23 0.0 0.0 0.0 0.1635097646561702 0.0 24 0.0 0.0 0.0 0.17453289485770976 0.0 25 0.0 0.0 0.0 0.18371883669232605 0.0 26 0.0 0.0 0.0 0.18371883669232605 0.0 27 0.0 0.0 0.0 0.20025353199463541 0.0 28 0.0 0.0 0.0 0.20209072036155867 0.0 29 0.0 0.0 0.0 0.20209072036155867 0.0 30 0.0 0.0 0.0 0.21127666219617497 0.0 31 0.0 0.0 0.0 0.21311385056309823 0.0 32 0.0 0.0 0.0 0.23699729933310063 0.0 33 0.0 0.0 0.0 0.24618324116771692 0.0 34 0.0 0.0 0.0 0.2627179364700263 0.0 35 0.0 0.0 0.0 0.28292700850618213 0.0 36 0.0 0.0 0.0 0.3362054711469567 0.0 37 0.0 0.0 0.0 0.4501111498961989 0.0 38 0.0 0.0 0.0 0.5676912053792875 0.0 39 0.0 0.0 0.0 0.6264812331208319 0.0 40 0.0 0.0 0.0 0.7458984769708438 0.0 41 0.0 0.0 0.0 0.8689900975547022 0.0 42 0.0 0.0 0.0 0.9241057485624001 0.0 43 0.0 0.0 0.0 1.0159651669085632 0.0 44 0.0 0.0 0.0 1.1482427293270379 0.0 45 0.0 0.0 0.0 1.3227756241847477 0.0 46 0.0 0.0 0.0 1.4293325494662967 0.0 47 0.0 0.0 0.0 1.502820084143227 0.0 48 0.0 0.0 0.0 1.5818191839209275 0.0 49 0.0 0.0 0.0 1.6791901673678602 0.0 50 0.0 0.0 0.0 1.796770222850949 0.0 51 0.0 0.0 0.0 1.9510940456725028 0.0 52 0.0 0.0 0.0 2.0686741011555916 0.0 53 0.0 0.0 0.0 2.171556649703294 0.0 54 0.0 0.0 0.0 2.2358582425456084 0.0 55 0.0 0.0 0.0 2.3369036027263874 0.0 56 0.0 0.0 0.0 2.4195770792379343 0.0 57 0.0 0.0 0.0 2.480204295346402 0.0 58 0.0 0.0 0.0 2.6051331042971837 0.0 59 0.0 0.0 0.0 2.643714060002572 0.0 60 0.0 0.0 0.0 2.7429222318164284 0.0 61 0.0 0.0 0.0 2.825595708327975 0.0 62 0.0 0.0 0.0 2.86233947566644 0.0 63 0.0 0.0 0.0 2.95236170564568 0.0 64 0.0 0.0 0.0 3.03687237052415 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGTGT 15 0.0022070368 70.05152 33 TGGGCGC 15 0.0022151112 69.98714 3 GGGTGTA 15 0.0022151112 69.98714 34 GGTGTAG 15 0.0022151112 69.98714 35 CTATTAG 15 0.0022151112 69.98714 7 ACACGTC 35 2.9269177E-7 59.98897 6 CACGTCT 30 8.282739E-6 58.322613 7 GGCGCTG 60 8.0035534E-11 52.53864 14 TGGCGCT 55 2.222805E-9 50.899734 13 GCGCTGC 35 2.052136E-5 50.0368 15 CTGCCGG 35 2.052136E-5 50.0368 18 ACGTCTG 35 2.0633533E-5 49.99081 8 GTATCAT 95 0.0 47.88593 57 CATTAAA 95 0.0 47.88593 61 TATCATT 95 0.0 47.88593 58 ATCATTA 95 0.0 47.88593 59 TCATTAA 95 0.0 47.88593 60 GCGCTGG 30 5.808728E-4 46.70101 15 CTGGCGC 60 4.800313E-9 46.658092 12 GTCTGGC 45 1.6582435E-6 46.65809 10 >>END_MODULE