FastQCFastQC Report
Wed 25 May 2016
SRR1780097_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780097_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54431
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT2456145.123183480002204No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG54399.992467527695615No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC16513.0331979937903033No Hit
ATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTCTT12442.285462328452536RNA PCR Primer, Index 39 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9371.7214454998070954No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC9141.6791901673678604No Hit
ATTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT4550.8359207069500836No Hit
CTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTC4470.8212232000146974No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGACGT4450.817548823280851No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTAAGCCGT3500.6430159284231413No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTAGTATGCCGT2570.47215741029927805No Hit
CTTATACACATCTCCGAGCCCACGAGACGCAAGTACATCTCGTATGCCGT2220.40785581745696387No Hit
TATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTCT2060.37846080358619166RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCAGT2000.3674376733846521No Hit
CTTTACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTC1880.345391412981573No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC1870.34355422461464974No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCACGTATGCCGT1830.3362054711469567No Hit
CCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG1790.32885671767926367No Hit
ATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTCAT1780.3270195293123404No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGA1770.3251823409454171No Hit
TTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTC1750.32150796421157063No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATATCGTATGCCGT1330.24434605280079366No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGAATGCCGT1290.23699729933310063No Hit
CTTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG1250.22964854586540756No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGAACATCTCGTATGCCGT1190.21862541566386803No Hit
CTTATACACATCTCCGAGACCACGAGACGCATGTACATCTCGTATGCCGT1140.20943947382925174No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCAG1140.20943947382925174No Hit
TCTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT1100.20209072036155867No Hit
CTTAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG1060.19474196689386564No Hit
GCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG910.16718414139001672No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGACG720.13227756241847477No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACAACTCGTATGCCGT710.1304403740515515No Hit
TCCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC710.1304403740515515No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTAGTATGCCG670.12309162058385846No Hit
CTTATAAACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT670.12309162058385846No Hit
TCTTATACACATCTCCGAGCCCACGAGCCGCATGTACATCTCGTATGCCG670.12309162058385846No Hit
CTTATACACATCTCCGAGCCAACGAGACGCATGTACATCTCGTATGCCGT660.1212544322169352No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATG640.11758005548308868No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTAAATCTCGTATGCCGT630.11574286711616544No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATGTACATCTCGTATGCCGT600.11023130201539563No Hit
CTTATACACATCTCAGAGCCCACGAGACGCATGTACATCTCGTATGCCGT600.11023130201539563No Hit
CTTATACACATCTCCGAGCACACGAGACGCATGTACATCTCGTATGCCGT550.10104536018077934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCC150.002209222470.0340456
AACTGCT150.002209222470.0340452
TATGCTA150.002209222470.0340453
CAACTGC150.002209222470.0340451
AGCTAGA150.002209222470.0340455
TTCTGAA150.002209222470.0340452
TATGATT150.002209222470.0340453
TAATGCT150.002209222470.0340452
TCTACAG150.002209222470.0340450
ACTTCAG150.002209222470.0340450
AGTCTTA150.002209222470.0340448
GCATCTA150.002209222470.0340429
GACTTCA150.002209222470.0340449
CTGAATG207.842055E-570.03403554
TCTGAAT207.842055E-570.03403553
TGAATGA207.842055E-570.03403555
CTCGAAT550.069.9696739
AAACTAT150.002217304769.9696738
TCGAATG600.069.9696740
TATCGTA301.0090662E-769.9696738
Produced by FastQC (version 0.11.5)