FastQCFastQC Report
Wed 25 May 2016
SRR1780096_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780096_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184117
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5491929.828315690566328No Hit
CTTATACACATCTGACGCTGCCGACGACGATACACGTGTAGATCTCGGTG7210.39159882031534293No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAAC207.910971E-570.023
TATGCGC207.910971E-570.03
ATGTACC150.002221929470.050
GACCTTC150.002221929470.01
GCCGTAT1650.057.2727354
TCGCCGT1750.056.052
GTCGCCG1750.056.051
CGTATCA1750.056.056
TGCGCGT509.749783E-1055.9999965
ATGCGCG252.385748E-455.9999964
GACGATT252.385748E-455.99999615
GTAGATC1800.054.44444738
CGCGTCT452.6870111E-854.4444477
GTATCAT1650.053.03030857
ATCTCGG1800.052.50000442
AGATCTC1800.052.50000440
CCGATAG200.006942813352.531
TTTCAAG200.006942813352.513
TGACCTT200.006942813352.548
CGAGCTA200.006942813352.523
Produced by FastQC (version 0.11.5)