FastQCFastQC Report
Wed 25 May 2016
SRR1780096_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780096_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184117
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT3298117.913066148155792No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG65633.56458121737808No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC26661.4479923092381475No Hit
ATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTCTT18140.9852430791290321Illumina PCR Primer Index 3 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12170.6609927383131378No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC10110.5491073610801828No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGACGT5240.28460163917508974No Hit
CTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTC5230.2840585062759006No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT4800.26070379161076923No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCAGT2950.16022420526078526No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGAAGACATCTCGTATGCCGT2940.15968107236159615No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGA2800.15207721177294872No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCAGCCATCTCGTATGCCGT2750.1493615472770032No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC2670.1450164840834904No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGCCATCTCGTATGCCGT2660.14447335118430127No Hit
TATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTCT2650.14393021828511218No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATATCGTATGCCGT2560.13904202219241027No Hit
TTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTC2530.13741262349484296No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGAAGCAATCTCGTATGCCGT2490.13524009189808656No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG2430.13198129450295193No Hit
CTTTACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTC2380.12926563000700642No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTAAGCCGT2240.12166176941835899No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG2070.11242851013214424No Hit
CTTATACACATCTCCGAGACCACGAGACTCGAAGCCATCTCGTATGCCGT2070.11242851013214424No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGAATGCCGT2010.10916971273700962No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACAT207.902912E-570.01441
ATCTTGG150.002220116870.01441
CGTAATC207.913556E-569.99538448
AACTCGT353.6925485E-969.99538437
GACGACT150.002222510569.99538446
CAACTCG550.063.63216836
AGGGGGG37200.061.4341270
ACGTATG401.0611984E-861.2459640
CGAATGC700.059.99604441
CACGTAT352.9635157E-759.99604439
CTCGCAG1100.057.28450828
TCTTTAT1350.057.048771
CGAAGAC800.056.88669630
GTCTTCA1550.056.4478949
CGTCTTA509.749783E-1055.99630748
TCGAATG950.055.25951440
TGCATGA2450.054.2821355
ACTCGCA1200.052.510827
AATCCGG200.006937166752.51079625
GAGTAAG200.006937166752.5107961
Produced by FastQC (version 0.11.5)