FastQCFastQC Report
Wed 25 May 2016
SRR1780095_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780095_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70795
Sequences flagged as poor quality0
Sequence length76
%GC83

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4285860.53817359983049No Hit
CTTATACACATCTGACGCTGCCGACGACGATACACGTGTAGATCTCGGTG4960.7006144501730348No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1190.16809096687619182No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG780.11017727240624337No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTATG150.002216261370.068
AGGTGGA150.002216261370.041
TCTCGGA150.002216261370.043
CGGTGGA150.002216261370.046
ATGCTAG150.002216261370.020
CTCGGAG150.002216261370.044
GGTGGAT150.002216261370.042
TGTAATT150.002216261370.042
TGTAATA150.002216261370.026
CGCAAAC150.002216261370.026
TCTGGCG308.299432E-658.33333211
AAACCCA200.00692523852.555
CGGAGGT200.00692523852.546
TTTCTCG200.00692523852.541
TAATGCT200.00692523852.518
TGTAGGT200.00692523852.537
GGAAGAG200.00692523852.563
TGGCGCT200.00692523852.513
AATGCTA200.00692523852.519
TGACGCA200.00692523852.513
Produced by FastQC (version 0.11.5)