##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780095_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 70795 Sequences flagged as poor quality 0 Sequence length 76 %GC 83 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.396567554205806 32.0 27.0 32.0 13.0 32.0 2 28.811441485980648 32.0 32.0 32.0 13.0 32.0 3 28.861176636768132 32.0 32.0 32.0 13.0 32.0 4 28.24052546083763 32.0 27.0 32.0 13.0 32.0 5 29.002584928314146 32.0 32.0 32.0 13.0 32.0 6 32.932904866162865 37.0 32.0 37.0 13.0 37.0 7 32.53012218377004 37.0 32.0 37.0 13.0 37.0 8 32.91516350024719 37.0 37.0 37.0 13.0 37.0 9 32.9223673988276 37.0 32.0 37.0 13.0 37.0 10-11 32.818934953033406 37.0 32.0 37.0 13.0 37.0 12-13 32.51755067448266 37.0 32.0 37.0 13.0 37.0 14-15 33.45121830637757 37.0 37.0 37.0 17.5 37.0 16-17 33.43087082421075 37.0 34.5 37.0 20.0 37.0 18-19 32.78577583162652 37.0 32.0 37.0 13.0 37.0 20-21 33.31441485980648 37.0 34.5 37.0 17.5 37.0 22-23 33.39145419874285 37.0 34.5 37.0 20.0 37.0 24-25 32.98809237940532 37.0 34.5 37.0 13.0 37.0 26-27 33.407747722296776 37.0 34.5 37.0 17.5 37.0 28-29 33.25003884455117 37.0 37.0 37.0 13.0 37.0 30-31 33.193311674553286 37.0 34.5 37.0 13.0 37.0 32-33 33.26075994067378 37.0 37.0 37.0 13.0 37.0 34-35 33.30418814888057 37.0 37.0 37.0 17.5 37.0 36-37 33.885888833957196 37.0 37.0 37.0 24.5 37.0 38-39 34.00362313722721 37.0 37.0 37.0 24.5 37.0 40-41 33.49048661628646 37.0 37.0 37.0 17.5 37.0 42-43 33.379977399533864 37.0 37.0 37.0 22.0 37.0 44-45 33.155328766155804 37.0 37.0 37.0 13.0 37.0 46-47 33.34728441274101 37.0 34.5 37.0 17.5 37.0 48-49 33.91151917508299 37.0 37.0 37.0 24.5 37.0 50-51 34.20204110459778 37.0 37.0 37.0 27.0 37.0 52-53 33.30704852037573 37.0 37.0 37.0 17.5 37.0 54-55 33.44071615227064 37.0 37.0 37.0 17.5 37.0 56-57 33.316279398262594 37.0 37.0 37.0 17.5 37.0 58-59 33.02257927819761 37.0 37.0 37.0 13.0 37.0 60-61 32.74455116886786 37.0 34.5 37.0 13.0 37.0 62-63 32.48639028179956 37.0 32.0 37.0 13.0 37.0 64-65 32.32992442969136 37.0 32.0 37.0 13.0 37.0 66-67 32.47102902747369 37.0 32.0 37.0 13.0 37.0 68-69 32.57380464722085 37.0 32.0 37.0 13.0 37.0 70-71 32.53592767850837 37.0 32.0 37.0 13.0 37.0 72-73 32.85038491418885 37.0 32.0 37.0 13.0 37.0 74-75 33.20401158273889 37.0 37.0 37.0 13.0 37.0 76 33.79160957694752 37.0 37.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 1.3840116327322534 11101 2 -0.3571065009833845 11101 3 -0.1274009117266104 11101 4 0.548913224932825 11101 5 0.3868142733068183 11101 6 1.2995079770050282 11101 7 0.43391131741051936 11101 8 0.05204105482781074 11101 9 1.6447382884150272 11101 10-11 0.8190416308166419 11101 12-13 0.5072054216225297 11101 14-15 2.27439029758159 11101 16-17 0.1333487025064386 11101 18-19 0.7017721847137963 11101 20-21 0.5558314171659049 11101 22-23 1.5250588670148844 11101 24-25 1.023875208176122 11101 26-27 1.2345910605110788 11101 28-29 0.3351112991148568 11101 30-31 1.2111232926808526 11101 32-33 -0.5134670076446568 11101 34-35 -1.0182859944264777 11101 36-37 1.0323058067442688 11101 38-39 -0.11841230245808987 11101 40-41 -1.090564297282782 11101 42-43 2.055676843943239 11101 44-45 0.34317827274862367 11101 46-47 0.39163828951441815 11101 48-49 1.013122248101105 11101 50-51 0.3491187041016772 11101 52-53 1.783864233268659 11101 54-55 0.6800244692071047 11101 56-57 0.2723086487257689 11101 58-59 0.422292716647668 11101 60-61 0.47525558163098225 11101 62-63 1.0669697940124863 11101 64-65 -0.20599200129535689 11101 66-67 0.03969888726636128 11101 68-69 0.8109804551808253 11101 70-71 -0.6811033681749947 11101 72-73 -0.8892526504353953 11101 74-75 -0.7925413842555358 11101 76 0.17655711933534235 11102 1 1.3116902041608256 11102 2 1.7402149275880454 11102 3 0.2993848025591035 11102 4 -0.006443917924318754 11102 5 1.4350285590211023 11102 6 0.7352222627193115 11102 7 -0.9143029683037653 11102 8 -0.293494659457906 11102 9 0.2277740027007411 11102 10-11 0.6043094879594975 11102 12-13 -0.16690172123461622 11102 14-15 1.2801938690101622 11102 16-17 -0.2501334403507087 11102 18-19 1.953557898999513 11102 20-21 -1.449079297119809 11102 22-23 0.15630886701488578 11102 24-25 0.5368216367475469 11102 26-27 0.38860891765394 11102 28-29 1.7427005848291444 11102 30-31 0.4553197212522804 11102 32-33 -0.041592007644659645 11102 34-35 0.5562675770020888 11102 36-37 0.09346652102998831 11102 38-39 1.3065876975419073 11102 40-41 -0.3499392972827806 11102 42-43 0.472641129657525 11102 44-45 0.037821129891476346 11102 46-47 0.29342400380012634 11102 48-49 0.03410439095824813 11102 50-51 0.8942079898159605 11102 52-53 1.1396678046972255 11102 54-55 -0.4137255307928953 11102 56-57 0.7798979344400578 11102 58-59 -0.29958228335232917 11102 60-61 -0.09929798979759141 11102 62-63 -1.83079806313037 11102 64-65 -0.5452777155810722 11102 66-67 -0.03039039844792768 11102 68-69 0.42303402660940037 11102 70-71 -1.4860140824607058 11102 72-73 1.004497349564602 11102 74-75 -0.26888066996982474 11102 76 -0.6466571663789438 11103 1 1.972694065164685 11103 2 1.922454309827426 11103 3 0.5905044936787966 11103 4 2.4120889006085022 11103 5 -0.0777127537202098 11103 6 1.6711295986266492 11103 7 1.209249155248358 11103 8 2.0207910548278107 11103 9 1.5999747749015114 11103 10-11 0.012031495681512183 11103 12-13 0.9894689351360455 11103 14-15 1.252008540824832 11103 16-17 0.9927237025064386 11103 18-19 0.17981272525433667 11103 20-21 0.7598010117605014 11103 22-23 1.4482007589067791 11103 24-25 2.4271691270950413 11103 26-27 2.085520114565135 11103 28-29 1.9596890018175586 11103 30-31 1.2402618061943684 11103 32-33 1.1448944788418274 11103 34-35 2.1578119785464906 11103 36-37 1.1602618878253494 11103 38-39 -0.5069258159716057 11103 40-41 1.6311417837982987 11103 42-43 0.22206197907837577 11103 44-45 0.5374350295053816 11103 46-47 1.7277869381630637 11103 48-49 0.5642202210740734 11103 50-51 0.20849370410167722 11103 52-53 -0.9614904964610673 11103 54-55 -0.29210390917126716 11103 56-57 1.7752647298068496 11103 58-59 4.177166476679872E-4 11103 60-61 2.231590041090442 11103 62-63 1.7101285777962687 11103 64-65 0.19603502573167475 11103 66-67 2.1634319953744736 11103 68-69 0.7083622119375832 11103 70-71 0.8742175777709491 11103 72-73 0.5963892414564924 11103 74-75 1.7492660481768993 11103 76 1.4012192814975037 11104 1 1.1812872737578957 11104 2 -0.8627154753423589 11104 3 0.5953554985297984 11104 4 -2.545638057118456 11104 5 -0.8455575215649773 11104 6 -1.5658766383795886 11104 7 -0.26961432361512294 11104 8 -0.9279268938901382 11104 9 0.7288729037996404 11104 10-11 0.03178201543202874 11104 12-13 0.5400579857250989 11104 14-15 -1.1450808562645634 11104 16-17 -0.9431737333909993 11104 18-19 -1.6684201229785138 11104 20-21 -0.4758192238597374 11104 22-23 -1.1872808765748601 11104 24-25 -2.222519022593108 11104 26-27 -2.1223800933350745 11104 28-29 -1.166090623962063 11104 30-31 -1.3758158098832496 11104 32-33 -1.2646689307215766 11104 34-35 -0.443366122631609 11104 36-37 -1.7285115009480378 11104 38-39 -0.7113610204068053 11104 40-41 -0.6935290408725265 11104 42-43 -2.348970591954199 11104 44-45 -2.116757624687274 11104 46-47 -1.010605300229173 11104 48-49 -1.1523424954886394 11104 50-51 -0.09919860359062938 11104 52-53 -1.0266325616031367 11104 54-55 -1.8752639923313552 11104 56-57 -0.24171378717166903 11104 58-59 -1.781633565403613 11104 60-61 -1.881715572215171 11104 62-63 -2.663398795731105 11104 64-65 -0.9463766166799701 11104 66-67 -1.4062145742720986 11104 68-69 -0.5483945448191747 11104 70-71 -1.25121554766217 11104 72-73 -0.10319495812770185 11104 74-75 -0.9908586919478424 11104 76 -2.2224813422031175 11105 1 -1.895635803165181 11105 2 -0.22168983431671663 11105 3 -1.3533624501881505 11105 4 -0.7251252366056349 11105 5 -1.973762649770105 11105 6 -0.540235612738563 11105 7 -3.0901271441279405 11105 8 -0.851003816967058 11105 9 0.7545139294406695 11105 10-11 -0.6733461896961757 11105 12-13 -0.12660868094157252 11105 14-15 -1.7861064972902057 11105 16-17 0.0568262666090078 11105 18-19 -1.783804738363127 11105 20-21 -1.4886397366802484 11105 22-23 -0.8026654919594733 11105 24-25 -1.6968779969520824 11105 26-27 -0.4428929138478992 11105 28-29 -2.153270111141552 11105 30-31 -1.568123502190943 11105 32-33 -0.7005663666190145 11105 34-35 -0.4177250969905799 11105 36-37 0.707385934949393 11105 38-39 -0.6857199947657833 11105 40-41 0.6782658309223422 11105 42-43 -0.9515346945182941 11105 44-45 -1.8218858298154785 11105 46-47 -0.20291299253686645 11105 48-49 -0.49849634164248613 11105 50-51 -0.394070398462425 11105 52-53 -0.731760766731341 11105 54-55 -1.0932127102800777 11105 56-57 0.21982467436679087 11105 58-59 -0.3970181807882298 11105 60-61 -0.7150489055485068 11105 62-63 -1.176219308551616 11105 64-65 -0.6130432833466379 11105 66-67 -0.9703171383746643 11105 68-69 0.1567336603090297 11105 70-71 -0.36660016304679033 11105 72-73 -0.10319495812770185 11105 74-75 0.12452592343677082 11105 76 -0.35068647040824885 11106 1 -0.18314262258689595 11106 2 1.4733456266761884 11106 3 1.0148863223159417 11106 4 0.45981748025197433 11106 5 0.09825044351958567 11106 6 0.6026994663667296 11106 7 -0.4370993208873557 11106 8 -0.1281451153849531 11106 9 0.04965850118098558 11106 10-11 -0.40602485854505943 11106 12-13 -0.47085308901576894 11106 14-15 0.8598291273688261 11106 16-17 0.896979021655369 11106 18-19 0.054165801735074126 11106 20-21 0.6120149278042035 11106 22-23 -0.02879751596383784 11106 24-25 -0.3002604301217531 11106 26-27 -0.7451296841697683 11106 28-29 -0.3281200838638654 11106 30-31 -0.04984745199999452 11106 32-33 0.4562803327808709 11106 34-35 -0.29388439868179717 11106 36-37 0.6962020833400118 11106 38-39 1.0278642932865907 11106 40-41 0.8372947452704125 11106 42-43 1.2677779077730307 11106 44-45 0.16365699615287355 11106 46-47 -0.6868191572940887 11106 48-49 0.8252898012925911 11106 50-51 0.6340256189952953 11106 52-53 -0.10410119226325776 11106 54-55 1.5543595755900839 11106 56-57 -0.12496528744443935 11106 58-59 -0.24426313441615832 11106 60-61 1.0061731348224683 11106 62-63 0.8306001131614167 11106 64-65 1.2966675731727264 11106 66-67 0.5766004830110418 11106 68-69 0.6643714126276308 11106 70-71 1.0665695041654288 11106 72-73 0.5768377750965215 11106 74-75 0.6466208497870198 11106 76 -0.16519819981359518 11107 1 -1.7930717006010788 11107 2 0.13728452465764107 11107 3 -0.2764393732650703 11107 4 -0.8276893391697406 11107 5 0.20572452971707378 11107 6 -3.6171586896616397 11107 7 -1.7567938107946084 11107 8 -2.825362791326036 11107 9 -0.7583065833798415 11107 10-11 -0.6477051640551537 11107 12-13 -1.8060958604287514 11107 14-15 -1.0937988049825123 11107 16-17 -2.0457378359551015 11107 18-19 -0.9120098665682548 11107 20-21 -0.4373576853981973 11107 22-23 -0.9821526714466557 11107 24-25 -1.5943138943879802 11107 26-27 -0.4428929138478992 11107 28-29 -0.8583983162697564 11107 30-31 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0.3688074373512009 11108 10-11 0.9344006733698365 11108 12-13 0.029146910984231056 11108 14-15 0.04068019119861077 11108 16-17 1.5565534897404802 11108 18-19 0.8094849506712407 11108 20-21 1.207759608655266 11108 22-23 1.2584365265893567 11108 24-25 0.3273991443463302 11108 26-27 0.850614996681287 11108 28-29 -1.4025881689702508 11108 30-31 -0.6881453243404252 11108 32-33 0.38181224767449606 11108 34-35 0.6103709204671404 11108 36-37 0.2600318705740605 11108 38-39 0.7193536549887156 11108 40-41 -0.5775988717508653 11108 42-43 0.48054386521983616 11108 44-45 -0.283151514485418 11108 46-47 0.21743616185484882 11108 48-49 0.8040132055479106 11108 50-51 0.9850894487825244 11108 52-53 0.4490902970984436 11108 54-55 0.6713808521858269 11108 56-57 0.44950279766193546 11108 58-59 1.0323326102646888 11108 60-61 0.08064121992885731 11108 62-63 0.25613202805504187 11108 64-65 -0.05439625661450975 11108 66-67 0.9808558021599794 11108 68-69 0.14309481688295023 11108 70-71 0.768697163739894 11108 72-73 -0.6146515866056106 11108 74-75 0.5721527646806308 11108 76 -1.1013684125795535 11109 1 0.6291505216211419 11109 2 0.7338657212388391 11109 3 1.1628768660511675 11109 4 1.6919687804883807 11109 5 1.3270920510845947 11109 6 -0.48382535632830326 11109 7 1.015855761854965 11109 8 0.5763466103833679 11109 9 1.069043843970583 11109 10-11 -0.13061114696113663 11109 12-13 0.7426220882891954 11109 14-15 0.8976541864704757 11109 16-17 0.9371681469508815 11109 18-19 0.36149440693601775 11109 20-21 1.3985397504992392 11109 22-23 0.8896422003482201 11109 24-25 1.5193613192872277 11109 26-27 1.7425771716221874 11109 28-29 1.2903196324481883 11109 30-31 1.6489705149030698 11109 32-33 1.169519103466456 11109 34-35 0.20706122779574798 11109 36-37 0.690291917855383 11109 38-39 1.3176988086530272 11109 40-41 1.6278384804950008 11109 42-43 1.642482399498796 11109 44-45 2.2806782727486237 11109 46-47 1.7680271784033081 11109 48-49 1.0134694703233222 11109 50-51 0.8084937041016786 11109 52-53 1.4237947888242175 11109 54-55 0.6640522469848875 11109 56-57 0.9830725376146603 11109 58-59 0.08930660553655656 11109 60-61 1.5054639149643165 11109 62-63 1.070789238456932 11109 64-65 1.2801191098157574 11109 66-67 0.9664349983774727 11109 68-69 0.8071610107363796 11109 70-71 1.1282716318250081 11109 72-73 1.382275127342382 11109 74-75 1.3168336157444642 11109 76 1.4168348971131195 11110 1 0.028633725755508266 11110 2 -0.02789138470431496 11110 3 0.022308390598972494 11110 4 -0.2868425890206616 11110 5 -0.6553368894838805 11110 6 1.444856814214333 11110 7 1.883039224387261 11110 8 1.625442217618506 11110 9 -1.4446512464686947 11110 10-11 0.7983294215143175 11110 12-13 -0.3845096946565434 11110 14-15 -0.20053702799980755 11110 16-17 -0.7630902509819322 11110 18-19 -0.2095650245885281 11110 20-21 -0.24177032702014145 11110 22-23 -0.9785748539153474 11110 24-25 -0.34058700112620244 11110 26-27 0.4500707116738667 11110 28-29 1.2453583921381153 11110 30-31 0.5988413159366672 11110 32-33 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-0.6071362202895045 21612 48-49 0.4245805814344408 21612 50-51 0.8065329197879478 21612 52-53 2.1309843313078716 21612 54-55 0.45882348881495005 21612 56-57 0.6941836487257689 21612 58-59 1.0396334029221777 21612 60-61 0.5564443071211755 21612 62-63 -1.0932630491247686 21612 64-65 0.7964589790967977 21612 66-67 0.800421926482052 21612 68-69 1.2555270238082699 21612 70-71 0.6047422200602952 21612 72-73 0.5084189181920564 21612 74-75 0.04232381182289657 21612 76 1.7880767271784777 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 9.0 12 28.0 13 64.0 14 130.0 15 226.0 16 392.0 17 633.0 18 703.0 19 736.0 20 769.0 21 757.0 22 827.0 23 967.0 24 1076.0 25 1155.0 26 1333.0 27 1509.0 28 1635.0 29 1915.0 30 2107.0 31 2618.0 32 3230.0 33 4272.0 34 6591.0 35 11265.0 36 25847.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 79.22028391835582 6.934105515926266 3.7022388586764605 10.143371707041458 2 72.80598912352568 6.95246839466064 13.515078748499187 6.7264637333145 3 73.16053393601243 7.350801610283213 12.257927819761283 7.230736633943074 4 72.59898577542977 7.646236209794753 11.054765301654118 8.700012713121353 5 71.70421639946323 10.493678932127976 13.22692280528286 4.575181863125927 6 77.16832410441857 12.645496666289976 5.574076166798508 4.612103062492937 7 75.11688678578996 9.698425030016244 6.576735645172683 8.607952539021117 8 75.56183346281517 12.421781199237234 6.282929585422699 5.733455752524896 9 74.27925700967583 5.696729995056148 6.3577936294936075 13.666219365774419 10-11 75.94039127056995 8.734373896461614 8.97238505544177 6.352849777526662 12-13 75.20940744402853 7.649551522000141 8.742142806695389 8.398898227275938 14-15 76.49057136803447 8.095910728158769 7.116321774136591 8.297196129670175 16-17 76.10848223744615 8.327565506038562 7.121971890670245 8.441980365845046 18-19 74.19202192276182 8.359465491425826 8.80371217899822 8.644800406814136 20-21 76.15566620757849 8.086308577886076 7.658297135996045 8.099728078539394 22-23 76.11379495437467 7.9738395909257855 7.226601124389073 8.685764330310478 24-25 75.44989688391671 9.07139023081052 7.266858773342373 8.21185411193039 26-27 76.47208390719355 8.972701910513118 7.502913444220787 7.052300738072535 28-29 76.25276331866627 8.27900866604985 7.901149116797446 7.567078898486443 30-31 75.59185806706783 8.913891007712518 8.317795293386446 7.176455631833206 32-33 75.18751853996865 9.108951450002118 7.9675956662381875 7.735934343791053 34-35 75.7278261975054 9.021372169564787 7.881428954840168 7.369372678089642 36-37 76.45741810630994 8.161117624623904 8.697893859562386 6.683570409503764 38-39 76.49007324118739 7.898323998672195 8.903359771730456 6.708242988409953 40-41 76.54940072181769 8.572820951076016 8.080544117750923 6.797234209355378 42-43 75.74972101761473 8.50931589281426 8.890709533428447 6.850253556142556 44-45 75.10346775902254 7.639663818066247 9.386962356098595 7.869906066812628 46-47 75.76294768661407 7.3565036831957284 8.831900783252937 8.048647846937262 48-49 76.1379768202332 7.41794913446666 9.572777546278314 6.871296499021817 50-51 76.54125673604588 7.554259158550453 8.68499671584657 7.219487389557099 52-53 75.04749662756286 7.879849423330909 9.143365656936627 7.9292882921695895 54-55 75.53640793841373 7.905925559714669 8.375591496574616 8.182075005296985 56-57 75.43594488272393 7.99144001299536 8.225215235646836 8.347399868633863 58-59 74.80789332429302 8.815718846229908 8.791705511766533 7.5846823177105405 60-61 75.00088283694355 8.010509290975994 8.791643418627153 8.196964453453305 62-63 74.82272008136512 8.548282291784384 8.89436659509549 7.734631031755 64-65 74.89970618148944 8.77994688665386 8.822324556447056 7.498022375409651 66-67 74.9738678419075 9.811565951916828 7.444133683645507 7.770432522530157 68-69 75.03955137441027 9.810153402830748 7.370681131169309 7.779614091589683 70-71 75.14143447399088 9.81883674118021 7.267012748525621 7.77271603630328 72-73 75.66796386673917 9.372329380522224 7.107290923601743 7.852415829136855 74-75 76.31121721074118 8.942268303363326 6.935007698501264 7.811506787394235 76 77.3911937958215 7.7792374736901575 6.948623412580695 7.880945317907644 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 1.0 6 1.0 7 0.5 8 1.0 9 1.0 10 1.0 11 0.5 12 0.0 13 3.5 14 6.5 15 6.0 16 10.5 17 15.0 18 24.0 19 34.0 20 39.0 21 43.0 22 42.0 23 54.0 24 80.5 25 94.0 26 135.0 27 197.5 28 222.5 29 226.0 30 226.5 31 301.5 32 409.0 33 442.0 34 510.0 35 624.0 36 719.0 37 768.0 38 755.0 39 890.0 40 1011.0 41 1033.5 42 1083.0 43 1148.0 44 1314.0 45 1437.0 46 1459.0 47 1299.5 48 1075.0 49 957.5 50 905.0 51 827.5 52 697.0 53 681.0 54 718.0 55 659.0 56 546.0 57 503.5 58 515.0 59 455.0 60 364.5 61 324.0 62 314.0 63 301.5 64 258.5 65 213.0 66 189.0 67 180.0 68 168.0 69 147.5 70 141.0 71 143.0 72 133.0 73 115.5 74 102.5 75 97.0 76 103.0 77 100.0 78 88.5 79 86.0 80 92.0 81 92.0 82 94.5 83 103.0 84 106.5 85 119.5 86 140.0 87 151.0 88 155.0 89 165.0 90 201.0 91 239.0 92 247.0 93 277.0 94 353.0 95 474.0 96 549.0 97 696.0 98 1296.0 99 22740.0 100 43731.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.002825058266826753 5 0.0 6 0.00423758740024013 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0014125291334133765 20-21 0.003531322833533442 22-23 0.0014125291334133765 24-25 0.0014125291334133765 26-27 0.003531322833533442 28-29 0.002118793700120065 30-31 0.0014125291334133765 32-33 0.002825058266826753 34-35 0.002825058266826753 36-37 0.002825058266826753 38-39 0.002118793700120065 40-41 0.002118793700120065 42-43 0.002825058266826753 44-45 0.0 46-47 7.062645667066883E-4 48-49 7.062645667066883E-4 50-51 7.062645667066883E-4 52-53 7.062645667066883E-4 54-55 0.0 56-57 7.062645667066883E-4 58-59 0.0014125291334133765 60-61 7.062645667066883E-4 62-63 0.00423758740024013 64-65 0.00423758740024013 66-67 0.0014125291334133765 68-69 0.0014125291334133765 70-71 0.003531322833533442 72-73 0.002118793700120065 74-75 0.002825058266826753 76 0.005650116533653506 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 70795.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.877392471219718 #Duplication Level Percentage of deduplicated Percentage of total 1 76.01159488878372 18.149586835228476 2 9.772834831992427 4.6669962567977965 3 6.1878845243729295 4.432516420651176 4 3.774254614292475 3.604774348470937 5 1.7747278750591577 2.118793700120065 6 0.9938476100331283 1.4238293664806838 7 0.49692380501656414 0.8305671304470654 8 0.26620918125887366 0.5085104880288156 9 0.18930430667297682 0.4068083904230525 >10 0.4910080454330336 2.0947807048520373 >50 0.023663038334122102 0.35595734162017095 >100 0.011831519167061051 0.8687054170492265 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.005915759583530526 60.53817359983049 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 42858 60.53817359983049 No Hit CTTATACACATCTGACGCTGCCGACGACGATACACGTGTAGATCTCGGTG 496 0.7006144501730348 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 119 0.16809096687619182 No Hit GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 78 0.11017727240624337 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005650116533653507 0.0 2 0.0 0.0 0.0 0.005650116533653507 0.0 3 0.0 0.0 0.0 0.005650116533653507 0.0 4 0.0 0.0 0.0 0.02542552440144078 0.0 5 0.0 0.0 0.0 0.04096334486898792 0.0 6 0.0 0.0 0.0 0.04661346140264143 0.0 7 0.0 0.0 0.0 0.052263577936294936 0.0 8 0.0 0.0 0.0 0.05791369446994844 0.0 9 0.0 0.0 0.0 0.06215128187018857 0.0 10 0.0 0.0 0.0 0.06215128187018857 0.0 11 0.0 0.0 0.0 0.06215128187018857 0.0 12 0.0 0.0 0.0 0.06215128187018857 0.0 13 0.0 0.0 0.0 0.06215128187018857 0.0 14 0.0 0.0 0.0 0.06497634013701532 0.0 15 0.0 0.0 0.0 0.06497634013701532 0.0 16 0.0 0.0 0.0 0.06497634013701532 0.0 17 0.0 0.0 0.0 0.06497634013701532 0.0 18 0.0 0.0 0.0 0.06497634013701532 0.0 19 0.0 0.0 0.0 0.0791016314711491 0.0 20 0.0 0.0 0.0 0.08051416060456247 0.0 21 0.0 0.0 0.0 0.08333921887138922 0.0 22 0.0 0.0 0.0 0.09463945193869623 0.0 23 0.0 0.0 0.0 0.10452715587258987 0.0 24 0.0 0.0 0.0 0.13136520940744403 0.0 25 0.0 0.0 0.0 0.13701532594109753 0.0 26 0.0 0.0 0.0 0.1398403842079243 0.0 27 0.0 0.0 0.0 0.15255314640864467 0.0 28 0.0 0.0 0.0 0.17232855427643196 0.0 29 0.0 0.0 0.0 0.20057913694469948 0.0 30 0.0 0.0 0.0 0.206229253478353 0.0 31 0.0 0.0 0.0 0.22741719047955364 0.0 32 0.0 0.0 0.0 0.2669680062151282 0.0 33 0.0 0.0 0.0 0.2952185888833957 0.0 34 0.0 0.0 0.0 0.33476940461897026 0.0 35 0.0 0.0 0.0 0.42658379829083976 0.0 36 0.0 0.0 0.0 0.5311109541634296 0.0 37 0.0 0.0 0.0 0.7006144501730348 0.0 38 0.0 0.0 0.0 0.9619323398545095 0.0 39 0.0 0.0 0.0 1.2571509287379052 0.0 40 0.0 0.0 0.0 1.597570449890529 0.0 41 0.0 0.0 0.0 1.946465145843633 0.0 42 0.0 0.0 0.0 2.234621089059962 0.0 43 0.0 0.0 0.0 2.5326647362101844 0.0 44 0.0 0.0 0.0 2.8434211455611274 0.0 45 0.0 0.0 0.0 3.233279186383219 0.0 46 0.0 0.0 0.0 3.618899639805071 0.0 47 0.0 0.0 0.0 4.0101702097605765 0.0 48 0.0 0.0 0.0 4.281375803375945 0.0 49 0.0 0.0 0.0 4.520093226922805 0.0 50 0.0 0.0 0.0 4.775761000070626 0.0 51 0.0 0.0 0.0 5.097817642488876 0.0 52 0.0 0.0 0.0 5.384561056571791 0.0 53 0.0 0.0 0.0 5.662829295854227 0.0 54 0.0 0.0 0.0 5.935447418603009 0.0 55 0.0 0.0 0.0 6.246203827953951 0.0 56 0.0 0.0 0.0 6.527297125503213 0.0 57 0.0 0.0 0.0 6.788615015184688 0.0 58 0.0 0.0 0.0 7.0739459001341904 0.0 59 0.0 0.0 0.0 7.339501377215905 0.0 60 0.0 0.0 0.0 7.60505685429762 0.0 61 0.0 0.0 0.0 7.777385408574052 0.0 62 0.0 0.0 0.0 8.027403065188219 0.0 63 0.0 0.0 0.0 8.276008192668973 0.0 64 0.0 0.0 0.0 8.544388728017516 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTTATG 15 0.0022162613 70.0 68 AGGTGGA 15 0.0022162613 70.0 41 TCTCGGA 15 0.0022162613 70.0 43 CGGTGGA 15 0.0022162613 70.0 46 ATGCTAG 15 0.0022162613 70.0 20 CTCGGAG 15 0.0022162613 70.0 44 GGTGGAT 15 0.0022162613 70.0 42 TGTAATT 15 0.0022162613 70.0 42 TGTAATA 15 0.0022162613 70.0 26 CGCAAAC 15 0.0022162613 70.0 26 TCTGGCG 30 8.299432E-6 58.333332 11 AAACCCA 20 0.006925238 52.5 55 CGGAGGT 20 0.006925238 52.5 46 TTTCTCG 20 0.006925238 52.5 41 TAATGCT 20 0.006925238 52.5 18 TGTAGGT 20 0.006925238 52.5 37 GGAAGAG 20 0.006925238 52.5 63 TGGCGCT 20 0.006925238 52.5 13 AATGCTA 20 0.006925238 52.5 19 TGACGCA 20 0.006925238 52.5 13 >>END_MODULE