FastQCFastQC Report
Wed 25 May 2016
SRR1780095_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780095_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70795
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT2419834.18037997033689No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG55807.881912564446641No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC17272.4394378134049015No Hit
ATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTCTT12711.7953245285684019TruSeq Adapter, Index 3 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8901.2571509287379052No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC7981.1271982484638745No Hit
ATTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT5390.76135320290981No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGACGT4180.5904371777667915No Hit
CTTATACACATCTCCGAGCCCACGAGACAACTAGGCATCTCGTATGCCGT3990.5635991242319373No Hit
CTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC3770.5325234832968431TruSeq Adapter, Index 3 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTAAGCCGT3060.43223391482449325No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATATCGTATGCCGT2570.3630199872872378No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCAGT2210.31216893848435623No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGA2010.2839183558160887No Hit
TATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTCT1910.26979306448195495RNA PCR Primer, Index 3 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCACGTATGCCGT1880.2655554770817148No Hit
TTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC1870.26414294794830145TruSeq Adapter, Index 3 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC1710.2415424818136874No Hit
CTTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG1680.2373048944134473No Hit
CTTATACACATCTCCGAGACCACGAGACCACTAGGCATCTCGTATGCCGT1440.20340419521152625No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGAATGCCGT1430.20199166607811286No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTAGTATGCCGT1410.19916660781128612No Hit
ATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTCAT1320.18645384561056572Illumina PCR Primer Index 3 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC1260.17797867081008548RNA PCR Primer, Index 3 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG1210.1709160251430186No Hit
TCTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT1150.1624408503425383No Hit
TCTTATACACATCTCCGAGCCCACGAGCCCACTAGGCATCTCGTATGCCG1120.15820326294229817No Hit
CTTATACACATCTCCGAGCCCACGAGACCACAAGGCATCTCGTATGCCGT1040.14690302987499118No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCAG1040.14690302987499118No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCAACTCGTATGCCGT980.1384278550745109No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTAGGAATCTCGTATGCCGT950.13419026767427078No Hit
CTTAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG860.12147750547355038No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGACG790.11158980153965677No Hit
CTTATACACATCTCCGAGCCAACGAGACCACTAGGCATCTCGTATGCCGT780.11017727240624337No Hit
CTTATACACATCTCCGAGCCCACGAGCCCACTAGGCATCTCGTATGCCGT760.10735221413941663No Hit
CATATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT740.10452715587258986No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC207.8805344E-569.9943541
TCTTGAA207.8805344E-569.99435456
ATCGAAT207.8805344E-569.99435439
ACCGGCG150.002216971269.9943547
AATCTCG252.8148625E-669.9943536
GTACTAA150.002216971269.994351
AACCCTA150.002216971269.994355
ATAATGG150.002216971269.994355
CTGAATG150.002216971269.9943554
AGTAATC150.002216971269.9943548
CTTAATA252.8148625E-669.994351
GCCGACA150.002216971269.9943546
CCGACAT150.002216971269.9943547
CCGGCGC150.002216971269.9943548
ACGGGGG453.765308E-1062.21719470
AGGGGGG27800.061.18210670
ATTATAC1300.059.2259861
TCTTTAT1300.059.2259861
CTGATTG308.3034065E-658.3286254
TTAATAC308.3034065E-658.328622
Produced by FastQC (version 0.11.5)