FastQCFastQC Report
Wed 25 May 2016
SRR1780094_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780094_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188769
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3152116.698186672599842No Hit
CTTATACACATCTGACGCTGCCGACGACGATACACGTGTAGATCTCGGTG3490.18488205160805005No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCT150.002221882370.0010660
TACGCAC150.002221882370.0010628
GACACTC150.00222421969.982513
GCCGTAT900.054.44526754
GAACTTT200.006942664752.50079311
GCAACAT200.006942664752.50079314
TAGGTAC200.006942664752.50079366
GTCTCGG200.006942664752.50079342
TCAATGC200.006942664752.50079315
GTTCGGG200.006942664752.50079319
CACTAGA200.006942664752.50079317
AACGCTG200.006942664752.50079369
GGTCTCG200.006942664752.50079341
GGATTCC200.006942664752.50079341
GCGTCGG200.006942664752.5007938
TGCCGGC200.006942664752.50079319
CCCTAGT200.00694994552.486891
GATCTCG1100.050.90986341
TGGTCGC1100.050.90986349
ATCTCGG1050.050.0007542
Produced by FastQC (version 0.11.5)