FastQCFastQC Report
Wed 25 May 2016
SRR1780094_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780094_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188769
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT170149.013132452892158No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG45142.3912824669304813No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC13450.7125110584894766No Hit
ATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTCTT8720.4619402550206867TruSeq Adapter, Index 11 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6460.3422172072744995No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC6400.3390387192812379No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT4020.21295869554852756No Hit
CTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTC2780.14726994368778773No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCAACATCTCGTATGCCGT2720.1440914556945261Illumina PCR Primer Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTAAGCCGT2680.14197246369901836No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGACGT2470.13084775572260277No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCGG150.00222028270.013781
TGCGATG150.00222028270.0137829
ACCCCGG150.002222617269.9952311
CGTAATC308.356474E-658.3293648
AGGGGGG21700.057.8992870
CTTTATA1300.056.549592
TAAACGT252.3835825E-456.0110220
CGCCCAA252.3835825E-456.0110230
GGAGCTA200.006937679452.5103341
TAGTGCG200.006937679452.51033426
CAGCATG1000.052.4964254
GGCGCGT200.006944954452.4964262
CCAGTTC200.006944954452.4964236
AATCTCG352.081626E-549.99659336
TCGAATG352.081626E-549.99659340
GCCGTAA352.081626E-549.99659346
GCCGTCA3950.049.61687546
TCTCGAA506.167829E-848.99666238
TTTATAC1550.047.4286883
CGTCATC4100.046.94802548
Produced by FastQC (version 0.11.5)