FastQCFastQC Report
Wed 25 May 2016
SRR1780093_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780093_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111168
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3023427.19667530224525No Hit
CTTATACACATCTGACGCTGCCGACGACGATACACGTGTAGATCTCGGTG3330.2995466321243523No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTA150.002220002569.9968539
TGCGCGT150.002220002569.996855
AATCTCG150.002220002569.9968520
GTCCCAT207.89896E-569.9968526
GTGCGCG150.002220002569.996854
TTAGAGT200.006936844452.4976455
TAGCGCC200.006936844452.4976437
GTTAGAG200.006936844452.4976454
TGTGCGG200.006936844452.497643
GTTAACA200.006936844452.4976438
AGCGCCT200.006936844452.4976438
GGCTAAC200.006936844452.497641
TATCCGC200.006936844452.4976440
TCTGGCG305.857685E-446.6645711
TCGCCGT855.638867E-1141.17461852
GTCGCCG855.638867E-1141.17461851
CGTATCA855.638867E-1141.17461856
GTAGATC855.638867E-1141.17461838
GTATCAT855.638867E-1141.17461857
CTCGGCT350.001251158739.99820323
Produced by FastQC (version 0.11.5)