FastQCFastQC Report
Wed 25 May 2016
SRR1780093_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780093_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111168
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT1769915.920948474381117No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG39823.5819660333909034No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC15621.4050805987334485No Hit
ATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTCTT10140.91213298791019RNA PCR Primer, Index 30 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC7260.6530656303972366No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5930.5334268854346574No Hit
ATTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT4450.40029504893494533No Hit
CTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTC3030.27256044905008636No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATATCGTATGCCGT2290.2059945308002303No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGACGT2270.20419545192861252No Hit
TATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTCT1590.14302677029360966No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCAGT1450.13043321819228557No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC1380.12413644214162348No Hit
CTTTACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTC1170.1052461139896373No Hit
TTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTC1130.10164795624640183No Hit
CCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG1120.10074841681059296No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAA150.002219606470.044
CGGAAAA150.002219606470.012
GATACAT207.8972E-570.032
GGCACGG150.002219606470.014
ACGGGTT150.002219606470.013
CCGTATT150.002219606470.047
ACGGAGA150.002219606470.017
ACTACGC150.002219606470.08
TGGATAC150.002219606470.030
CTTTATA1200.070.02
GTCTTAT207.8972E-570.049
GCCGTAT150.002219606470.046
TCTTTAT1300.064.615391
AACTCGT401.05537765E-861.2537
AGGGGGG20250.059.80246770
CGTCTTA308.330604E-658.33333248
TAAGCCG601.8189894E-1258.33333243
TCTGGAA308.330604E-658.33333232
AAGCCGT553.45608E-1157.27272444
TTTATAC1500.056.03
Produced by FastQC (version 0.11.5)